PMID: 9525680Apr 3, 1998Paper

cis-Acting sequences required for simian foamy virus type 1 vectors

Journal of Virology
M WuA Mergia

Abstract

We have constructed a series of vectors based on simian foamy virus type 1 (SFV-1) to define the minimum cis-acting elements required for gene transfer. To characterize these vectors, we inserted the coding sequence of the bacterial lacZ gene linked to the cytomegalovirus immediate-early gene promoter. Introduction of a deletion mutation in the leader region between the 5' long terminal repeat and the start of the gag gene at position 1659 to 1694 completely abrogated gene transfer by the SFV-1 vector. Deletion of 39 nucleotides from position 1692 to 1731 in the leader region resulted in a significant reduction in the transducing-particle titer. Furthermore, we have identified a second cis-acting element located at the 3' end of the pol gene between position 6486 and 6975 to be critical for SFV-1 vector transduction. These results identify the two important cis-acting elements required for SFV-1 vector construction, and the finding of a cis-acting element in the pol gene is unique among retroviruses.

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Citations

Dec 1, 2001·Expert Opinion on Biological Therapy·S PandyaV Planelles
Mar 22, 2002·Journal of Virology·Martin HeinkeleinAxel Rethwilm
Jan 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Grant D TrobridgeDavid W Russell
Oct 14, 2011·Viruses·Dirk Lindemann, Axel Rethwilm
Sep 3, 2016·Viruses·Martin V Hamann, Dirk Lindemann
Mar 27, 2001·Journal of Virology·D CainM O McClure
Jun 26, 2007·Acta Pharmacologica Sinica·Zhe MaYun-qi Geng
Feb 18, 2011·Journal of Virology·Maximilian J HartlBirgitta M Wöhrl
Jan 15, 2009·The Journal of General Virology·Tatiana WiktorowiczAxel Rethwilm
Sep 12, 2009·Expert Opinion on Biological Therapy·Grant D Trobridge
Mar 9, 2007·Journal of Virology·Jacqueline Roy, Maxine L Linial
Oct 12, 2016·Viruses·Lizna M AliFarah Mustafa
Apr 10, 1999·Journal of Virology·M Wu, A Mergia
Apr 25, 2003·Journal of Virological Methods·Hang T T PhungHiroomi Akashi
Sep 2, 2011·Cold Spring Harbor Protocols·Jeonghae Park, Ayalew Mergia

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