cis-Acting signal for inheritance of imprinted DNA methylation patterns in the preimplantation mouse embryo.

Molecular and Cellular Biology
C Y HowellJ R Chaillet

Abstract

The inheritance of gametic methylation patterns is a critical event in the imprinting of genes. In the case of the imprinted RSVIgmyc transgene, the methylation pattern in the unfertilized egg is maintained by the early mouse embryo, whereas the sperm's methylation pattern is lost in the early embryo. To investigate the cis-acting requirements for this preimplantation stage of genomic imprinting, we examined the fate of different RSVIgmyc methylation patterns, preimposed on RSVIgmyc and introduced into the mouse zygote by pronuclear injection. RSVIgmyc methylation patterns with a low percentage of methylated CpG dinucleotides, generated by using bacterial cytosine methylases with four-base recognition sequences, were lost in the early embryo. In contrast, methylation was maintained when all CpG dinucleotides were methylated with the bacterial SssI (CpG) methylase. This singular maintenance of RSVIgmyc methylation preimposed with SssI methylase appears to be specific to the early, undifferentiated embryo; differentiated NIH 3T3 fibroblasts transfected with methylated versions of RSVIgmyc maintained all methylation patterns, independent of the level of preimposed methylation. The methylation pattern of the RSVIgmyc allele in adul...Continue Reading

References

Jan 1, 1975·Cytogenetics and Cell Genetics·A D Riggs
Jan 1, 1988·Annual Review of Genetics·D Solter
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·R SteinH Cedar
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T H Bestor, V M Ingram
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C L StewartR Jaenisch
Jun 1, 1994·Mutation Research·J R Chaillet
Jan 1, 1993·EXS·J Singer-Sam, A D Riggs
May 15, 1995·Genes & Development·J R ChailletP Leder
Apr 1, 1995·Nature Genetics·K D TremblayM S Bartolomei
Apr 22, 1993·Nature·S RainierA P Feinberg
Mar 19, 1996·Proceedings of the National Academy of Sciences of the United States of America·F M Boyce, N L Bucher
Sep 6, 1996·Cell·A WeissH Cedar
Aug 1, 1997·Trends in Genetics : TIG·J A YoderT H Bestor
Sep 1, 1997·Molecular and Cellular Biology·K Weichman, J R Chaillet
Apr 12, 2005·Science·Uwe H F Bunz

❮ Previous
Next ❯

Citations

Dec 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Frederic ChedinChih-Lin Hsieh
Dec 17, 2008·Biology of Reproduction·Yong ChengKeith E Latham
Nov 13, 2004·Biology of Reproduction·Zhiming HanKeith E Latham
Jun 30, 2000·Journal of Molecular Biology·V P MiaoE U Selker
Jul 29, 2004·Journal of Cellular Physiology·Jan Cerny, Peter J Quesenberry
Jun 18, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S LauM W McBurney
Mar 9, 2002·Molecular and Cellular Biology·Bonnie ReinhartJ Richard Chaillet
Jul 27, 2000·Molecular and Cellular Biology·J LiuL S Weinstein
Jul 16, 2010·The Journal of Biological Chemistry·Celeste Holz-Schietinger, Norbert O Reich
Sep 29, 1998·Genome Research·M ConstânciaW Reik
Jan 20, 2005·Biology of the Cell·Keith E Latham

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.