Cis and Trans Cooperativity of E-Cadherin Mediates Adhesion in Biomimetic Lipid Droplets

Biophysical Journal
Lea-Laetitia PontaniJasna Brujic

Abstract

The regulation of cell-cell adhesion is important in cell motility, tissue growth, and for the mechanical integrity of tissues. Although the role of active cytoskeleton dynamics in regulating cadherin interactions is crucial in vivo, here we present a biomimetic emulsion system to characterize the passive E-cadherin-mediated adhesion between droplets. The visualization of a three-dimensional assembly of lipid droplets, functionalized with extracellular E-cadherin domains, reveals a hierarchy of homophilic interactions. First, the high interfacial tension of droplets facilitates trans cadherin-cadherin adhesion, which is strong enough to stabilize looser than random close packing configurations. Second, fluorescence enhancement shows that adding clustering agents, such as calcium or chelating ligands, favor the lateral cis adhesion of the already bound cadherin pairs over the clustering of monomer cadherin on the surface. Finally, above a threshold cadherin and calcium concentration, the cis and trans protein interactions become strong enough to trigger and promote droplet fusion. While E-cadherin is not known to participate in cellular fusion, this mechanism is general because replacing calcium with cholesterol to cluster the c...Continue Reading

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Citations

Aug 1, 2018·Soft Matter·Anaïs GiustinianiWiebke Drenckhan
Mar 17, 2020·Soft Matter·Iaroslava GolovkovaLea-Laetitia Pontani
Jan 18, 2017·Cold Spring Harbor Perspectives in Biology·René Marc Mège, Noboru Ishiyama
Jul 11, 2020·Advanced Biosystems·Solveig M BarteltSeraphine V Wegner
Jun 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Xinzhi LiDapeng Bi
Mar 5, 2021·Proceedings of the National Academy of Sciences of the United States of America·Connor J ThompsonDaniel K Schwartz
Mar 17, 2021·Soft Matter·Iaroslava GolovkovaLea-Laetitia Pontani
Dec 7, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·L PinonJ Fattaccioli
May 12, 2020·Biochimica Et Biophysica Acta. Biomembranes·K Sabrina LynnMichael Koval

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