PMID: 781520Jun 15, 1976

Cis-dominant regulatory mutations affecting the formation of glucose-repressible alcohol dehydrogenase (ADHII) in Saccharomyces cerevisiae

Molecular & General Genetics : MGG
M Ciriacy

Abstract

The formation of ADHII in Saccharomyces cerevisiae is regulated by carbon catabolite repression. There are two genes involved in the formation of ADHII: ADR2, the structural gene as identified by electrophoretic variants and ADR1, possibly a regulatory gene. A new genetic element involved in the regulation of ADHII was identified by three allelic mutants insensitive to strong glucose repression. They were called ADR3c (wild type designation ADR3) and found to be tightly linked to the structural gene, ADR2. The alcohol dehydrogenase found in ADR3c mutants could not be distinguished electrophoretically from the ADHII of the glucose-sensitive wild type, ADR3. Dominance relations between ADR3c and ADR3 were established in diploids heterozygous for ADR3 and the two alleles of ADR2 (ADR2-S: slow ADHII, ADR2-F: fast ADHII). During growth on 10% glucose, an ADR3c adr2-F/ADR3 ADR2-Sheterozygous diploid formed only the fast ADHII variant wheras an ADR3c ADR2-S/ADR3 ADR2-F heterozygote produced only the slow form. This was taken as evidence of the cis-dominance of all ADR3c alleles. The cis-effect of ADR3c was also demonstrated in glucose-derepressed diploids. The ADR3c mutations do not only cause glucose-insensitive ADHII frmation, but a...Continue Reading

References

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Citations

Jul 7, 1977·Molecular & General Genetics : MGG·F K Zimmermann, I Scheel
Feb 27, 1978·Molecular & General Genetics : MGG·M Ciriacy
Oct 18, 1976·Molecular & General Genetics : MGG·P Haussmann, F K Zimmermann
Oct 25, 1978·Molecular & General Genetics : MGG·M J Hynes
Oct 1, 1982·Current Genetics·M Ciriacy, D Breilmann
Sep 1, 1984·Current Genetics·A K McCann, J A Barnett
May 1, 1984·Current Genetics·H M Sealy-Lewis, R A Lockington
Jan 1, 1981·Molecular & General Genetics : MGG·M Ciriacy, V M Williamson
Feb 28, 1977·Molecular & General Genetics : MGG·F K ZimmermannP Haubetamann
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A Sledziewski, E T Young
Jun 6, 2000·Microbiology and Molecular Biology Reviews : MMBR·V Contamine, M Picard
Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·C Wills
Jan 1, 1987·Critical Reviews in Biotechnology·R E Subden
Sep 1, 2005·Yeast·James A Barnett, Karl-Dieter Entian
Nov 21, 1977·Biochemical and Biophysical Research Communications·E Petrochilo, J Verdiĕre
Dec 25, 2007·Journal of Biology·Markus RalserSylvia Krobitsch
May 16, 2008·FEMS Yeast Research·Olga de SmidtJacobus Albertyn
Aug 18, 1983·Nature·D W RussellE T Young
Apr 26, 2019·Scientific Reports·Kobi Simpson-Lavy, Martin Kupiec

Related Concepts

Alcohol Oxidoreductases
Alleles
Enzyme Repression
Cistron
Genes, Dominant
Genes, Regulator
Anhydrous Dextrose
Saccharomyces cerevisiae

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