PMID: 8592113Feb 1, 1996Paper

Cis-element/cytoplasmic protein interaction within the 3'-untranslated region of the GLUT1 glucose transporter mRNA

Journal of Neurochemistry
K J DwyerW M Pardridge

Abstract

The posttranscriptional regulation of glucose transporter GLUT1 gene expression may be mediated by specific interactions of cytosolic proteins and regulatory cis-elements within the untranslated regions (UTRs) of the GLUT1 mRNA. These putative cis/trans interactions were examined in the present studies with RNase T1 protection assays using 32P-labeled GLUT1 3'-UTR prepared from transcription plasmids and cytosolic proteins from C6 rat glioma cells. RNase T1 mapping studies localized a cis-element to nucleotides 2,170-2,207 on the bovine GLUT1 mRNA 3'-UTR. Ultraviolet cross-linking of RNA/protein complexes identified two complexes having molecular masses of 88 and 44 kDa. Competition studies with synthetic RNA and oligodeoxynucleotides showed the 88-kDa complex reacted with nucleotides 2,180-2,197 and that the 44-kDa complex reacted with sequences within nucleotides 1,717-2,132 of the bovine GLUT1 mRNA. The GLUT1 3'-UTR between nucleotides 2,100 and 2,300 was generated by polymerase chain reaction and subcloned at a unique Pfl/MI site within the 3'-UTR of a luciferase gene within the mammalian expression vector pGL2. Transfection of C6 rat glioma cells with the luciferase expression vector containing this portion of the GLUT1 3'...Continue Reading

Citations

Dec 18, 2002·The Journal of Biological Chemistry·Cinzia Dello RussoDouglas L Feinstein
Dec 4, 2012·International Journal of Molecular Sciences·Kaushik ShahThomas Abbruscato
Jan 5, 2000·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·E M Koehler-StecI A Simpson
Jun 7, 2000·Proceedings of the National Academy of Sciences of the United States of America·N Shi, W M Pardridge
Jan 14, 1999·Journal of Neurochemistry·I A SimpsonQ R Smith
Mar 10, 1999·The American Journal of Physiology·J Y KhanS U Devaskar
Apr 5, 2002·The Journal of Gene Medicine·Yun ZhangWilliam M Pardridge
Feb 23, 2020·Medical Hypotheses·Luana Lemos LeãoRenato Sobral Monteiro-Junior
Sep 24, 1999·Biochemical and Biophysical Research Communications·C Qi, P H Pekala
Aug 12, 1999·Biochemical and Biophysical Research Communications·B J HamiltonW F Rigby
Jan 8, 1999·Brain Research. Molecular Brain Research·R J Boado, W M Pardridge
Feb 29, 2000·Placenta·N P Illsley
Apr 17, 1999·Metabolism: Clinical and Experimental·B A BrucknerP W Stacpoole
Jul 13, 1999·American Journal of Kidney Diseases : the Official Journal of the National Kidney Foundation·J Z ZhangF Ismail-Beigi
Aug 5, 2009·General and Comparative Endocrinology·Olga HrytsenkoJames R Wright

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