Cisatracurium induces mast cell activation and pseudo-allergic reactions via MRGPRX2

International Immunopharmacology
Delu CheTao Zhang

Abstract

Pseudo-allergic reactions occur when patients receive muscle relaxants during perioperative anesthesia. These reactions may result in a serious threat to the patient's life, particularly to a child's life. Cisatracurium, a relatively new NMBA, has resulted in bronchospasms and cardiovascular collapse. However, the mechanisms underlying the anaphylactoid reactions caused by cisatracurium have not been fully elucidated. In the present study, the MRGPRX2-related pseudo-allergic reactions induced by cisatracurium were investigated using hindpaw swelling and extravasation assays in vivo and mast cell degranulation assays. Cisatracurium caused anaphylactoid reactions in wild-type mice. However, cisatracurium did not induce a similar phenomenon in KitW-sh/W-sh mice. Furthermore, mast cell-related G protein-coupled receptor B2-knockout mice did not display an inflammatory response upon treatment with cisatracurium. Cisatracurium induced LAD2 cell degranulation, leading to the dose-dependent release of β-hexosaminidase, histamine and TNF-α. However, cisatracurium only induced the release of low levels of these mediator LAD2 cells transfected with MRGPRX2 siRNA. Cisatracurium also stimulated intracellular Ca2+ influx in MRGPRX2-HEK293 ce...Continue Reading

Citations

May 12, 2020·Frontiers in Immunology·Brianna N CallahanHariharan Subramanian
Feb 11, 2020·Frontiers in Immunology·Christopher J OcchiutoHariharan Subramanian
Oct 20, 2020·The Journal of Allergy and Clinical Immunology·Helen KühnMarcus Maurer
Aug 11, 2020·The Journal of Investigative Dermatology·Maria ShtesselSarbjit S Saini
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Apr 2, 2021·Frontiers in Immunology·Maud A W HermansWillem A Dik
May 7, 2021·The Journal of Allergy and Clinical Immunology·Saptarshi RoyHydar Ali
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Aug 24, 2021·Frontiers in Immunology·Benjamin D McNeil
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