Cisgenesis and intragenesis in microalgae: promising advancements towards sustainable metabolites production

Applied Microbiology and Biotechnology
Janeth GalarzaVitalia Henríquez

Abstract

Microalgae are an economically important source of biomolecules and metabolites that can be exploited as feed, nutraceuticals and, potentially, as biofuels, among other biotechnological applications. Microalgae biotechnology involves both culture and metabolic pathways manipulation to obtain high-value products, such as omega-3 fatty acids and carotenoids. However, the introduction of genes and/or foreign regulatory sequences has caused public concern about the effect of genetically modified microalgae to achieve greater secondary metabolite accumulations. To placate these worries, we have focused on two cutting-edge concepts, cisgenesis and intragenesis in order to sustainably produce commercially relevant metabolites. This review provides updated background on current and future uses for microalgae molecular farming. We also discuss the development of genetic tools used in terrestrial plants to obtain genetically modified microalgae free of foreign DNA by means of (i) site-specific mutations, (ii) excision of selectable markers, (iii) zinc-finger nuclease and transcription activator-like effectors, and (iv) CRISPR/Cas9 systems. It is currently important to consider scientific debate not only from a technological standpoint bu...Continue Reading

References

Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·K L Kindle
Jun 12, 1996·Molecular & General Genetics : MGG·N FischerJ D Rochaix
May 16, 2002·Plant Physiology·Miri LapidotMichal Shapira
Dec 24, 2003·Trends in Biotechnology·Rosa León-BañaresEmilio Fernández
Aug 10, 2004·Applied Microbiology and Biotechnology·Otto Pulz, Wolfgang Gross
Mar 11, 2005·Expert Opinion on Biological Therapy·Scott E Franklin, Stephen P Mayfield
Jun 28, 2005·The FEBS Journal·Thierry TononIan A Graham
Sep 2, 2005·Plant Cell Reports·Tara L WalkerChris Collet
Oct 11, 2005·Applied Microbiology and Biotechnology·Jun-Chao HuangFeng Chen
Nov 22, 2005·Critical Reviews in Biotechnology·Sawraj SinghU C Banerjee
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Claire RemacleNathalie Bonnefoy
Mar 30, 2006·Journal of Bioscience and Bioengineering·Pauline SpolaoreArsène Isambert
Oct 3, 2006·Applied and Environmental Microbiology·Jens Steinbrenner, Gerhard Sandmann
Mar 14, 2007·Biotechnology Advances·Yusuf Chisti
Mar 27, 2007·Trends in Biotechnology·Evert Jacobsen, Henk J Schouten
Aug 19, 2007·Trends in Plant Science·Caius M RommensWilliam R Belknap
Dec 29, 2007·Advances in Experimental Medicine and Biology·Samuel P FletcherStephen P Mayfield
Apr 15, 2008·Médecine sciences : M/S·Jean-Paul CadoretAude Carlier
May 20, 2008·Trends in Plant Science·Henk J Schouten, Evert Jacobsen
Apr 30, 2009·Annual Review of Phytopathology·Jens Boch, Ulla Bonas
Jun 3, 2009·Molecular Phylogenetics and Evolution·Fabio RindiJuan M López-Bautista
Nov 17, 2009·Proceedings of the National Academy of Sciences of the United States of America·Patrick RömerThomas Lahaye
Nov 26, 2009·Science·Matthew J Moscou, Adam J Bogdanove
Jan 9, 2010·Science·Philippe Horvath, Rodolphe Barrangou
Feb 4, 2010·Nature Reviews. Genetics·Luciano A Marraffini, Erik J Sontheimer
Feb 9, 2010·Eukaryotic Cell·Randor RadakovitsMatthew C Posewitz
Jun 24, 2010·Current Opinion in Plant Biology·Adam J BogdanoveThomas Lahaye
Oct 15, 2010·Journal of Agricultural and Food Chemistry·Zhi-Wei Ye, Jian-Guo Jiang
Mar 24, 2011·Plant Biotechnology Journal·Anil Day, Michel Goldschmidt-Clermont
May 11, 2011·Marine Drugs·Carlos VílchezJosé M Vega

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Jun 4, 2020·Biotechnology for Biofuels·Jessica Varela VillarrealChristine Rösch

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