Cisplatin regulates cell autophagy in endometrial cancer cells via the PI3K/AKT/mTOR signalling pathway

Oncology Letters
Qiongyan LinHanzhen Xiong

Abstract

Endometrial cancer is the most common gynaecological malignancy encountered in developed countries and the second most common in the developing world. The five-year survival rate of patients with endometrial cancer diagnosed at a late stage is <30%. Therefore, it is critical to develop a suitable chemotherapeutic regimen for late-stage endometrial cancer. Cisplatin (CDDP) is a first-line chemotherapeutic drug for endometrial cancer chemotherapy. The present study investigated the molecular mechanism underlying the effect of CDDP on endometrial cancer from the perspective of cell autophagy. Ishikawa cells were treated with 10, 20, 40 or 80 µg/ml CDDP for 12, 24, 48 and 72 h. The cells were then harvested and subjected to cell proliferation assays. Based on the results, 20 µg/ml CDDP was selected as the treatment used for 12 and 24 h for the assays. To detect the effect of CDDP on Ishikawa cell autophagy, autophagosome formation was observed using a transmission electron microscope, and the expression level of autophagy-related gene microtubule-associated protein 1 light chain 3α, was examined using immunofluorescence microscopy. The results demonstrated that CDDP treatment promoted cell autophagy in Ishikawa cells. In addition, ...Continue Reading

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Citations

Dec 10, 2019·Anti-cancer Agents in Medicinal Chemistry·Yingying ChenMingqi Tan
Jun 30, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yingying ZhuGuixing Ren
Aug 12, 2018·International Journal of Molecular Sciences·Tom Van NyenFrédéric Amant
May 14, 2020·Autophagy·Laura Devis-JaureguiDavid Llobet-Navàs
Feb 10, 2021·Cell Biology International·Qin LiZhao Ma
Apr 21, 2021·Reproductive Sciences·Pooja PopliRamakrishna Kommagani
May 13, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Qiongyan LinQingping Jiang

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Methods Mentioned

BETA
hysterectomy
Assay
protein assay
transmission electron microscopy

Software Mentioned

SPSS

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