Cisplatin-resistant prostate cancer model: Differences in antioxidant system, apoptosis and cell cycle

International Journal of Oncology
Jaromir GumulecMichal Masarik

Abstract

Differences in the antioxidant system, apoptotic mechanism and in cell cycle between prostatic cell lines could partially elucidate the development of cisplatin resistance. The aim of this study was to identify the most characteristic parameter for a particular cell line and/or a particular cisplatin treatment using a general regression model and to assess whether it is possible to use measured parameters as markers of cisplatin resistance. This study integrates the results of viability, antioxidant, flow cytometric and quantitative PCR assays in order to characterize the resistance of prostate cancer to cisplatin. Cell growth using metabolic- (MTT) and impedance-based assays, the expression of key cell death signaling proteins (p53, Bax and Bcl-2), cell cycle, activity of antioxidant system-related proteins (superoxide dismutase, glutathione peroxidase, glutathione reductase and metallothionein) and free radical scavenging capacity assays [free radicals (FR), ferric reducing antioxidant power (FRAP), ABTS] were analyzed in the cell lines 22Rv1, PC-3 and PNT1A with respect to rising concentrations (0-150 µM) and different length of cisplatin treatment (12-72 h). The non-functional-p53 PC-3 cell line showed decreased BAX (p<0.05...Continue Reading

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Citations

Jun 14, 2014·Metallomics : Integrated Biometal Science·Monika HolubovaMichal Masarik
Nov 13, 2014·Metallomics : Integrated Biometal Science·Thiago de O AraujoReinaldo Calixto de Campos
Sep 3, 2016·BMC Complementary and Alternative Medicine·Sindiswa T Lukhele, Lesetja R Motadi
Jun 10, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Lawrence H LashAdam D Jankovich
Jun 4, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Katarzyna StarskaAnna Krześlak
Dec 27, 2018·International Journal of Molecular Sciences·Heng Wee TanYan-Ming Xu
Jun 24, 2014·Medical Oncology·Katarzyna StarskaMagdalena Bryś
Sep 17, 2015·Acta Biochimica Et Biophysica Sinica·Beiqin Yu, Jingwu Xie
Feb 2, 2020·Scientific Reports·Tomas VicarJan Balvan
Aug 4, 2016·Molecular Medicine Reports·Zhi-Yuan ChenXiu-Heng Liu
Oct 8, 2019·Toxicology Research·Tugce BoranBuket Alpertunga
Apr 13, 2018·Journal of Translational Medicine·Iman W AchkarFatima Mraiche
Mar 23, 2021·Scientific Reports·Miguel Angel Merlos RodrigoVojtech Adam
May 25, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Reinner O OmondiStephen O Ojwach

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