Cistanche deserticola extract increases bone formation in osteoblasts

The Journal of Pharmacy and Pharmacology
Te-Mao LiChih-Hsin Tang

Abstract

We investigated the effect of Cistanche deserticola Ma. (CD) on bone formation by cultured osteoblasts. The mineralized nodule formation assay was used to examine the in-vitro effects of CD on bone formation. Alkaline phosphatase (ALP), bone morphogenetic proteins (BMP)-2 and osteopontin (OPN) mRNA expression was analysed by quantitative real-time polymerase chain reaction. The mechanism of action of CD extract was investigated using Western blotting. The in-vivo anti-osteoporotic effect of CD extract was assessed in ovariectomized mice. CD extract had no effect on the proliferation, migration or wound healing of cultured osteoblasts, but increased ALP, BMP-2 and OPN mRNA and bone mineralization. Mitogen-activated protein kinase (MAPK) or nuclear factor (NF)-κB inhibitors reduced CD extract-induced bone formation and ALP, BMP-2 and OPN expression. However, CD extract did not affect osteoclastogenesis. In addition, CD extract prevented the bone loss induced by ovariectomy in vivo. CD may be a novel bone formation agent for the treatment of osteoporosis.

References

Mar 21, 1998·Journal of Ethnopharmacology·M C Lu
Sep 1, 2000·Science·G A Rodan, T J Martin
Jul 17, 2001·Immunology·R J van't Hof, S H Ralston
Oct 3, 2002·Nature Reviews. Drug Discovery·David Goltzman
Apr 12, 2003·Nature Reviews. Drug Discovery·Carilee BergPeter Kirkpatrick
Aug 23, 2003·Arthritis Research & Therapy·Nancy E Lane, Ariella Kelman
Sep 3, 2003·The Journal of Biological Chemistry·Huey-Chun HuangHua-Lin Wu
Dec 24, 2003·PLoS Biology·Unnur StyrkarsdottirKari Stefansson
Aug 31, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Aaron Schindeler, David G Little
Nov 14, 2006·The Journal of Biological Chemistry·Chih-Hsin TangWen-Mei Fu
May 23, 2008·European Journal of Pharmacology·Huei-Yann TsaiChih-Hsin Tang
Dec 3, 2008·Cytokine & Growth Factor Reviews·Jiake XuRen Xiang Tan
Sep 1, 2009·Journal of Cellular Biochemistry·Ernesto Canalis
Nov 17, 2009·Cytokine & Growth Factor Reviews·Charles C Hong, Paul B Yu
Apr 16, 2010·Annals of the New York Academy of Sciences·Brendan F BoyceLianping Xing
May 25, 2010·Biochimica Et Biophysica Acta·Subash C GuptaBharat B Aggarwal
Jun 16, 2010·The Biochemical Journal·Christopher C Rider, Barbara Mulloy
Aug 3, 2010·Rheumatology International·Pooneh Salari SharifBagher Larijani
Sep 2, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Fu-Hsiung LinBrian E Brigman
Apr 1, 2011·Lancet·Tilman D RachnerLorenz C Hofbauer
Jul 21, 2011·Nature Immunology·Jürgen Ruland
Sep 8, 2011·Journal of Cellular Biochemistry·Jung-Lye KimYoung-Hee Kang
Dec 14, 2011·Advances in Pharmacological Sciences·A S NazrunS Ima Nirwana

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Citations

Nov 29, 2014·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eunkuk ParkSeon-Yong Jeong
Mar 2, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Chun-Tao CheChristopher Wai Kei Lam
Oct 26, 2013·BMC Complementary and Alternative Medicine·Chi-Ming WuChih-Hsin Tang
Sep 23, 2016·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Eunkuk ParkSeon-Yong Jeong
Dec 12, 2012·The American Journal of Chinese Medicine·Tian WangWenyan Xie
Jan 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoxue XuXueqin Ma
May 6, 2017·BioMedicine·Min-Huan WuChih-Hsin Tang
Jan 19, 2018·Aging and Disease·Ningqun WangXianglan Jin
Aug 4, 2020·Chemical & Pharmaceutical Bulletin·Huibo LeiWeidong Zhang
Jun 21, 2019·International Journal of Molecular Sciences·Lingling YangXueqin Ma
Sep 12, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Shi-Lei ZhangTao Liu
Feb 2, 2021·Medicinal Research Reviews·Yuelin SongPengfei Tu

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