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Citrate and phosphate influence on green fluorescent protein thermal stability

Biotechnology Progress

Sep 25, 2010

Letícia C de Lencastre NovaesThereza C V Penna

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PubMed

Abstract

Protein structure and function can be regulated by no specific interactions, such as ionic interactions in the presence of salts. Green fluorescent protein (GFP) shows remarkable structural stability and high fluorescence; its stability can be directly related to its fluorescence output...read more

Mentioned in this Paper

Citrate Measurement
Phosphate Measurement
Fluorescent stain
Green Fluorescent Proteins
Denaturation
Citrate
Protein Denaturation
Orthophosphate
Salts
Phosphate ion
Paper Details
References
  • References16
  • Citations6
12
  • References16
  • Citations6
1

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Effect of polyethylene glycol on the thermal stability of green fluorescent protein

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Citrate and phosphate influence on green fluorescent protein thermal stability

Biotechnology Progress

Sep 25, 2010

Letícia C de Lencastre NovaesThereza C V Penna

PMID: 20865748

DOI: 10.1002/btpr.495

Abstract

Protein structure and function can be regulated by no specific interactions, such as ionic interactions in the presence of salts. Green fluorescent protein (GFP) shows remarkable structural stability and high fluorescence; its stability can be directly related to its fluorescence output...read more

Mentioned in this Paper

Citrate Measurement
Phosphate Measurement
Fluorescent stain
Green Fluorescent Proteins
Denaturation
Citrate
Protein Denaturation
Orthophosphate
Salts
Phosphate ion

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

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Examining the genomics, transcriptomics, proteomics and metabolomics of the human vascular endothelial cells at the single cell level will help build a single cell atlas of human vascular endothelium, which would give a unique ID card for each cell type, a three-dimensional map of how cell types work together to form tissues and insights into how changes in the map underlie health and disease of the vascular endothelium from young to old age. Disocver the latest research on the single cell atlas of human vascular endothelial cell atlas here.

Related Papers

Biotechnology Progress

Effect of polyethylene glycol on the thermal stability of green fluorescent protein

Biotechnology ProgressOctober 27, 2009
Letícia C de Lencastre NovaesThereza C V Penna
Paper Details
References
  • References16
  • Citations6
12
  • References16
  • Citations6
1

Download from

Publisher
PubMed
/papers/citrate-and-phosphate-influence-on-green/20865748