PMID: 4226177Aug 1, 1966

Citrate and the regulation of adipose-tissue phosphofructokinase

The Biochemical Journal
R M Denton, P J Randle

Abstract

1. Methods are described for the extraction, partial purification and assay of phosphofructokinase in rat epididymal adipose tissue. 2. The enzyme was inhibited by ATP at concentrations above 20mum and by citrate; the enzyme was activated by ADP, AMP, 3',5'-(cyclic)-AMP, phosphate and sulphate. 3. The enzyme lost activity on incubation at 25 degrees or during chromatography on DEAE-cellulose unless fluoride at a concentration of 4mm was present. 4. The significance of these results in relation to the role of glycolysis and citrate in lipogenesis is discussed.

Citations

Nov 12, 2013·Biopharmaceutics & Drug Disposition·Lisl K M ShodaBrett A Howell
May 14, 2014·The Journal of General Physiology·Christopher MulliganJoseph A Mindell
Aug 1, 1968·Metabolism: Clinical and Experimental·C Chlouverakis
Apr 29, 2015·Biology of the Cell·Doriane LorendeauSarah-Maria Fendt
May 9, 2012·Cell Metabolism·Deborah M Muoio, P Darrell Neufer
Aug 23, 1967·Biochemical and Biophysical Research Communications·G A Bray
Mar 1, 1968·European Journal of Biochemistry·E E Sel'kov
Jan 1, 1971·Archives of Biochemistry and Biophysics·R K RajG P Talwar
Nov 1, 1972·Journal of Neurochemistry·A Gromek, U Rafalowska
Mar 1, 1973·Journal of Dairy Science·Y T Yang, R L Baldwin
Jan 6, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Wing-Hang Tong, Tracey A Rouault
Nov 7, 1972·European Journal of Biochemistry·M OreviE Shafrir
Feb 24, 2015·The Biochemical Journal·Guy A RutterAida Martinez-Sanchez
Apr 29, 2014·Molecular Endocrinology·David J HodsonGuy A Rutter
Jan 1, 1971·Naunyn-Schmiedebergs Archiv Für Pharmakologie·R W Butcher
Jul 14, 2018·Genes & Nutrition·Samar H K TareenChris T Evelo
Jan 1, 1972·Naunyn-Schmiedeberg's Archives of Pharmacology·N Ofori-Nkansah, F von Bruchhausen
Jan 1, 1979·Acta Medica Scandinavica·T T Nielsen, N S Sørensen

Related Concepts

Adenine Nucleotides
Adenosine Triphosphate, Chromium Ammonium Salt
Fat Pad
Chromatography
Citrates
Fluorides
Glycolysis
Lipids
Orthophosphate
6-Phosphofructokinase

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