PMID: 7536931Apr 11, 1995Paper

Citrate modulates the regulation by Zn2+ of N-methyl-D-aspartate receptor-mediated channel current and neurotransmitter release

Proceedings of the National Academy of Sciences of the United States of America
N WestergaardA Schousboe

Abstract

The effect of the two metal-ion chelators EDTA and citrate on the action of N-methyl-D-aspartate (NMDA) receptors was investigated by use of cultured mouse cerebellar granule neurons and Xenopus oocytes, respectively, to monitor either NMDA-evoked transmitter release or membrane currents. Transmitter release from the glutamatergic neurons was determined by superfusion of the cells after preloading with the glutamate analogue D-[3H]aspartate. The oocytes were injected with mRNA isolated from mouse cerebellum and, after incubation to allow translation to occur, currents mediated by NMDA were recorded electrophysiologically by voltage clamp at a holding potential of -80 mV. It was found that citrate as well as EDTA could attenuate the inhibitory action of Zn2+ on NMDA receptor-mediated transmitter release from the neurons and membrane currents in the oocytes. These effects were specifically related to the NMDA receptor, since the NMDA receptor antagonist MK-801 abolished the action and no effects of Zn2+ and its chelators were observed when kainate was used to selectively activate non-NMDA receptors. Since it was additionally demonstrated that citrate (and EDTA) preferentially chelated Zn2+ rather than Ca2+, the present findings s...Continue Reading

References

Aug 1, 1989·Neurochemical Research·J Drejer, A Schousboe
Apr 19, 1984·Nature·S Y Assaf, S H Chung
Aug 1, 1994·Neuropharmacology·N L Harrison, S J Gibbons
Jan 7, 1993·Nature·T V Bliss, G L Collingridge

❮ Previous
Next ❯

Citations

Nov 20, 1998·Journal of Neuroscience Research·T Moos, E H Morgan
Nov 9, 2000·European Journal of Pharmacology·A A LarsonA K Spartz
Jun 24, 2000·Proceedings of the Society for Experimental Biology and Medicine·D J Rowe, D J Bobilya
May 20, 1999·Annual Review of Pharmacology and Toxicology·M AschnerW J Streit
Dec 19, 2000·Biochemical and Biophysical Research Communications·T SaitoH Fujita
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·C JacobB L Vallee
Jan 6, 2007·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Wing-Hang Tong, Tracey A Rouault
Sep 5, 2002·Journal of Neuroscience Research·Mari GårsethJohn-Anker Zwart
Apr 8, 2015·Neurochemical Research·Caroline M VossHelle S Waagepetersen
May 24, 2008·Autonomic Neuroscience : Basic & Clinical·Hui-Ling GaoZhan-You Wang
Sep 20, 2015·Brain : a Journal of Neurology·Katia HardiesUNKNOWN autosomal recessive working group of the EuroEPINOMICS RES Consortium
Oct 21, 1998·The Journal of Comparative Neurology·M K Christensen, C J Frederickson
Oct 28, 2005·Journal of Applied Toxicology : JAT·Nikolay V GoncharovAndrey S Radilov
Apr 5, 2001·American Journal of Physiology. Cell Physiology·A M PajorX Yao
Dec 26, 2018·Clinical Hemorheology and Microcirculation·Hoyoon LeeSehyun Shin
Feb 26, 2019·Journal of Neuroscience Research·Arne SchousboeUrsula Sonnewald
Sep 3, 2002·Journal of Neurophysiology·Dao-Qi ZhangDouglas G McMahon
Jan 7, 2017·Neurochemical Research·Niels WestergaardArne Schousboe
May 12, 2020·Frontiers in Cellular Neuroscience·Adam Armada-MoreiraSandra H Vaz
Mar 8, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yangzom D BhutiaVadivel Ganapathy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.