Citrate synthase 1 interacts with the citrate transporter of yeast mitochondria

Journal of Molecular Recognition : JMR
E V GrigorenkoP A Srere

Abstract

We have previously shown that citrate synthase binds to an intrinsic protein of the mitochondrial inner membrane (D'Souza and Srere, 1983). In this paper we present evidence that this citrate synthase binding protein is the citrate transporter. We have used citrate synthase 1 mutants of Saccharomyces cerevisiae and transformants containing citrate synthase inactivated by site-directed mutagenesis to study the effect of the CS1 protein upon mitochondrial function (Kispal and Srere). In the present study citrate uptake and oxidation were measured during state 3 conditions (presence of 200 microM ADP) in the mitochondria of several strains of Saccharomyces cerevesiae: a parental strain containing wild-type mitochondrial citrate synthase (CS1) and strains derived from a CS1 deficient strain in which the CS1 gene was disrupted by insertion of the LEU2 gene. These strains were generated from the CS1- cells by transformation with vectors encoding site-specific mutants of CS1 possessing very low levels of enzymatic activity. One such strain in this study was subsequently found to have undergone reversion to produce a strain which had activity very similar to wild type. Positive correlation between citrate uptake and the rate of citrate...Continue Reading

References

Jan 1, 1987·Annual Review of Biochemistry·P A Srere
Jun 1, 1986·Molecular and Cellular Biology·K S KimL Guarente
Mar 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L F Bisson, D G Fraenkel
Sep 1, 1983·Journal of Bacteriology·L F Bisson, D G Fraenkel

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Citations

Aug 31, 2013·The Biochemical Journal·Manuel Gutiérrez-Aguilar, Christopher P Baines
Feb 28, 1992·Biochemical and Biophysical Research Communications·L O Persson, P A Srere
Feb 14, 2019·Applied Microbiology and Biotechnology·Levente Karaffa, Christian P Kubicek
Jun 1, 2018·Nature Communications·Lee J Sweetlove, Alisdair R Fernie
Sep 25, 2019·Research : a Science Partner Journal·Alisdair R FernieLee J Sweetlove

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