PMID: 6412681Jul 1, 1983Paper

Citrate synthase from a Gram-positive bacterium. Purification and characterization of the Bacillus megaterium enzyme

The Biochemical Journal
M S RobinsonP D Weitzman

Abstract

Citrate synthase was purified to homogeneity from a Gram-positive bacterium (Bacillus megaterium) for the first time. The Mr of the native enzyme was determined to be 84 000 (S.E.M. +/- 5000). Sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and gel filtration in guanidinium chloride revealed a single protein species of Mr 40 300 (S.E.M. +/- 4400), indicating a dimeric enzyme. This dimeric structure was confirmed by cross-linking the native enzyme with dimethyl suberimidate and with glutaraldehyde, followed by electrophoretic analysis. The enzyme follows Michaelis-Menten kinetics with respect to both substrates, acetyl-CoA and oxaloacetate, and is sensitive to non-specific inhibition by a range of adenine nucleotides. In both molecular and catalytic properties the citrate synthase closely resembles the enzyme from eukaryotic sources and contrasts markedly with the larger, hexameric, enzyme from Gram-negative bacteria.

Citations

Dec 25, 2012·Chemistry & Biology·Johnson CheungDavid E Heinrichs
Jan 1, 1994·Society for Applied Bacteriology Symposium Series·R E MarquisS Y Shin
Apr 17, 2019·Scientific Reports·Shoki ItoTakashi Osanai
Aug 7, 1998·Biochimica Et Biophysica Acta·S G Andersson
Jun 1, 1996·Journal of Bacteriology·S Jin, A L Sonenshein
Jan 1, 1992·Applied and Environmental Microbiology·F J SchendelM C Flickinger
Aug 1, 1994·Journal of Bacteriology·S Jin, A L Sonenshein
Aug 1, 1987·Journal of Bacteriology·D O WoodD C Krause

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