Citrate synthase in Corynebacterium glutamicum is encoded by two gltA transcripts which are controlled by RamA, RamB, and GlxR

Journal of Biotechnology
Jan van OoyenL Eggeling

Abstract

Citrate synthase (CS) is located at a major branch point in the metabolism and is required for both tricarboxylic acid and glyoxylic acid cycle activity. Here we show that the CS gene gltA of Corynebacterium glutamicum is monocistronic, but that two transcripts are formed with their transcript initiation sites located 121 bp and 357 bp upstream of the translational start codon, respectively. Northern blot analyses revealed that during growth on acetate the short transcript prevails, whereas during growth on glucose the long transcript is dominant. Further Northern blots, reporter gene fusions, and CS activity measurements in mutants devoid of the transcriptional regulators RamA and RamB or with the global regulator GlxR overexpressed revealed a complex involvement of these regulators in gltA transcription. This was confirmed by demonstrating the direct interaction of isolated RamA, RamB and GlxR proteins with specific gltA promoter regions in vitro. The combined analyses point to an elaborate control of gltA transcript formation, which is possibly required as a dedicated mechanism to balance the total CS activity according to the physiological requirements.

References

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Citations

Dec 14, 2011·Applied Microbiology and Biotechnology·P Peters-WendischV F Wendisch
Oct 27, 2015·Applied Microbiology and Biotechnology·Koichi Toyoda, Masayuki Inui
Feb 1, 2011·Journal of Biotechnology·Haruhiko TeramotoHideaki Yukawa
Feb 1, 2011·Journal of Biotechnology·Miroslav Pátek, Jan Nešvera
May 30, 2015·BMC Microbiology·Ulrike Sorger-HerrmannVolker F Wendisch
May 29, 2018·Applied Microbiology and Biotechnology·Adnan ShahBernhard J Eikmanns
Nov 13, 2020·Frontiers in Bioengineering and Biotechnology·Michaela GrafRalf Takors

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