Citrate-tris(hydroxymethyl)aminomethane-mediated release of outer membrane sections from the cell envelope of a deep-rough (heptose-deficient lipopolysaccharide) strain of Escherichia coli O8.

Journal of Bacteriology
R T IrvinJ W Costerton

Abstract

A heptose-deficient lipopolysaccharide strain of Escherichia coli O8, strain F515, was found to release portions of its outer membrane when cells were exposed to 10 mM citrate buffer (pH 2.75) for 30 min and subsequently exposed to 100 mM tris(hydroxymethyl)aminomethane buffer (pH 8.00). The outer membrane component release was found to be composed of protein, lipopolysaccharide, phospholipid (cardiolipin, phosphatidylethanolamine, and phosphatidylglycerol), and alkaline phosphatase. The outer membrane component was released from the cell envelope in the absence of cell lysis, as no glucose-6-phosphate dehydrogenase activity or succinic dehydrogenase activity was detected. Morphologically, the outer membrane component appeared to consist of laminar fragments and vesicles which had an associated alkaline phosphatase activity.

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Citations

Dec 4, 2004·Bioprocess and Biosystems Engineering·Marie SvenssonSven-Olof Enfors
Dec 1, 1981·Archives of Microbiology·M S HindahlB J Wilkinson
Oct 1, 1982·Journal of Bacteriology·C S Fornari, S Kaplan
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Sep 1, 1982·Journal of Bacteriology·N KatsuiI Shibasaki
Apr 25, 1984·Biochimica Et Biophysica Acta·H H Bodemann, B Karsch

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