Citrinin reduces testosterone secretion by inducing apoptosis in rat Leydig cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
Shuqiang LiuDefu Hu

Abstract

A previous study has shown that CTN (Citrinin) inhibits mouse testosterone production. In this study, the mechanism by which testosterone production is inhibited by CTN in rat Leydig cells was investigated, and the morphological evidence of apoptosis, including nuclei fragmentation and phosphatidylserine (PS) exposure on cell surfaces, was clearly observed 36h after CTN exposure. The results showed that citrinin at 50 and 100μM significantly suppressed testosterone secretion by human chorionic gonadotropin (hCG) at 10IU/ml. Western blotting results showed that CTN induced formation of processed p53, caspase-9, and caspase-3 proteins in a dose-dependent manner; CTN also induced a dose-dependent increase in caspase-3 catalytic activity. Western blot assays also showed that CTN decreased expression of three key enzymes (P450scc, 3β-HSD-1, and StAR) of testosterone production. Taken together, these results suggested that CTN reduced testosterone secretion by inducing apoptosis in rat Leydig cells, a mechanism that might account for CTN stimulation of p53 expression followed by activation of multiple caspases.

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Citations

Mar 26, 2013·Journal of Biochemical and Molecular Toxicology·Jinwen LiuShuqiang Liu
Nov 1, 2016·Molecular Reproduction and Development·Ahmed BulldanGeorgios Scheiner-Bobis
May 30, 2019·Drug and Chemical Toxicology·Yasemin AydinMelike Erkan
Sep 16, 2016·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Joshua W SmithJohn W Erdman

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