Citrullinated histone 3 causes endothelial barrier dysfunction

Biochemical and Biophysical Research Communications
Jamie E MeeganSarah Y Yuan

Abstract

Circulating components of neutrophil extracellular traps (NETs), especially histones, are associated with tissue injury during inflammatory conditions like sepsis. Commonly used as a NET biomarker, citrullinated histone 3 (H3Cit) may also functionally contribute to the NET-associated inflammatory response. To this end, we sought to examine the role of H3Cit in mediating microvascular endothelial barrier dysfunction. Here we show that H3Cit can directly contribute to inflammatory injury by disrupting the microvascular endothelial barrier. We found that endothelial responses to H3Cit are characterized by cell-cell adherens junction opening and cytoskeleton reorganization with increased F-actin stress fibers. Several signaling pathways often implicated in the transduction of hyperpermeability, such as Rho and MLCK, did not appear to play a major role; however, the adenylyl cyclase activator forskolin blocked the endothelial barrier effect of H3Cit. Taken together, the data suggest that H3Cit-induced endothelial barrier dysfunction may hold promise to treat inflammatory injury.

Citations

Jun 12, 2019·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Jae Woong HwangSang Hag Lee
Sep 18, 2020·Xenotransplantation·Tao LiYi Wang
Jul 24, 2020·Journal of Thrombosis and Thrombolysis·Richard C Becker
Jul 21, 2020·Clinical and Translational Allergy·Pawel KucziaStanislawa Bazan-Socha
Jan 31, 2020·Frontiers in Immunology·Qiufang DengYongqing Li
Mar 24, 2020·Cellular & Molecular Immunology·Renato G S ChiriviJos M H Raats
Sep 24, 2020·Journal of Neuroinflammation·Nuria VillalbaSarah Y Yuan
Oct 22, 2020·International Journal of Molecular Sciences·Seung-Woo KimJa-Kyeong Lee
Nov 11, 2020·International Journal of Molecular Sciences·Takashi Obama, Hiroyuki Itabe
Oct 10, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Victor ChatterjeeSarah Y Yuan
Nov 27, 2020·Pulmonary Circulation·Pratap KarkiAnna A Birukova
Apr 26, 2021·Clinical Biochemistry·Mauro MoianaGabriela de Larrañaga
Jun 13, 2021·Molecular Cancer·Dongwei ZhuShengjun Wang
Jun 15, 2021·Frontiers in Immunology·Fadel Muhammad GarishahQuirijn de Mast
Nov 9, 2021·Molecular Neurobiology·Congqin LiYulong Bai

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.