Citrus limon extract: possible inhibitory mechanisms affecting testicular functions and fertility in male mice

Systems Biology in Reproductive Medicine
Nidhi Singh, Shio Kumar Singh

Abstract

The effect of oral administration of 50% ethanolic leaf extract of Citrus limon (500 and 1,000 mg/kg body weight/day) for 35 days on fertility and various male reproductive endpoints was evaluated in Parkes strain of mice. Testicular indices such as histology, 3β- and 17β-HSD enzymes activity, immunoblot expression of StAR and P450scc, and germ cell apoptosis by TUNEL and CASP- 3 expression were assessed. Motility, viability, and number of spermatozoa in the cauda epididymidis, level of serum testosterone, fertility indices, and toxicological parameters were also evaluated. Histologically, testes in extract-treated mice showed nonuniform degenerative changes in the seminiferous tubules. Treatment had adverse effects on steroidogenic markers in the testis and induced germ cell apoptosis. Significant reductions were noted in epididymal sperm parameters and serum level of testosterone in Citrus-treated mice compared to controls. Fertility of the extract-treated males was also suppressed, but libido remained unaffected. By 56 days of treatment withdrawal, alterations induced in the above parameters returned to control levels suggesting that Citrus treatment causes reversible suppression of spermatogenesis and fertility in Parkes mi...Continue Reading

References

Oct 1, 1990·Archives of Histology and Cytology·S K Singh, S K Rath
Dec 1, 1987·Archivum Histologicum Japonicum = Nihon Soshikigaku Kiroku·S K Singh, K Abe
Apr 14, 1999·Cell Death and Differentiation·A G Porter, R U Jänicke
Jan 1, 2003·Reproductive Toxicology·Shio K Singh, Sumana Chakravarty
Jul 1, 1957·American Journal of Clinical Pathology·S REITMAN, S FRANKEL
May 4, 2006·The Journal of Clinical Endocrinology and Metabolism·Thomas G TravisonJohn B McKinlay
Sep 4, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Raghav Kumar Mishra, Shio Kumar Singh
Dec 2, 2008·Contraception·Akanksha Singh, Shio Kumar Singh
Jun 26, 2009·Reproductive Biology and Endocrinology : RB&E·Imen HammamiClaire Mauduit
Feb 1, 1946·The Anatomical Record·M ABERCROMBIE
Jun 11, 2011·Journal of Biomedicine & Biotechnology·Lidianne Mayra Lopes CampêloRivelilson Mendes de Freitas
Apr 28, 2012·North American Journal of Medical Sciences·Ifeanyi Princewill OgbuewuIfeanyi Charles Okoli
Aug 13, 2014·The European Journal of Contraception & Reproductive Health Care : the Official Journal of the European Society of Contraception·Hari Prakash Verma, Shio Kumar Singh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

© 2022 Meta ULC. All rights reserved