CK2 is a novel negative regulator of NADPH oxidase and a neuroprotectant in mice after cerebral ischemia.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Gab Seok KimPak H Chan

Abstract

NADPH oxidase is a major complex that produces reactive oxygen species (ROSs) during the ischemic period and aggravates brain damage and cell death after ischemic injury. Although many approaches have been tested for preventing production of ROSs by NADPH oxidase in ischemic brain injury, the regulatory mechanisms of NADPH oxidase activity after cerebral ischemia are still unclear. In this study, we identified casein kinase 2 (CK2) as a critical modulator of NADPH oxidase and elucidated the role of CK2 as a neuroprotectant after oxidative insults to the brain. We found that the protein levels of the catalytic subunits CK2alpha and CK2alpha', as well as the total activity of CK2, are significantly reduced after transient focal cerebral ischemia (tFCI). We also found this deactivation of CK2 caused by ischemia/reperfusion increases expression of Nox2 and translocation of p67(phox) and Rac1 to the membrane after tFCI. Interestingly, we found that the inactive status of Rac1 was captured by the catalytic subunit CK2alpha under normal conditions. However, binding between CK2alpha and Rac1 was immediately diminished after tFCI, and Rac1 activity was markedly increased after CK2 inhibition. Moreover, we found that deactivation of CK2 ...Continue Reading

References

Apr 1, 1994·Stroke; a Journal of Cerebral Circulation·T ShishidoK Hirakawa
Jun 25, 1999·Cell Death and Differentiation·R Z Orlowski
Jan 13, 2000·Trends in Neurosciences·K K Wang
Mar 10, 2001·Stroke; a Journal of Cerebral Circulation·T MoriT Asano
Mar 13, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Flavio Meggio, Lorenzo A Pinna
May 2, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Yili Yang, Xiaodan Yu
Mar 25, 2004·Nature Reviews. Immunology·J David Lambeth
Jul 6, 2004·The European Journal of Neuroscience·Jordane MalaterreMyriam Cayre
Nov 30, 2004·Journal of Medicinal Chemistry·Mario A PaganoLorenzo A Pinna
Jan 29, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Atsushi SaitoPak H Chan
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Taku SugawaraPak H Chan
Apr 15, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Tatsuro NishiPak H Chan
Jun 15, 2005·Molecular Neurobiology·Atsushi SaitoPak H Chan
Oct 21, 2005·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Robert M BellMichael J Shattock
Nov 18, 2005·Biochemical and Biophysical Research Communications·Gab Seok KimByung Hee Han
Feb 25, 2006·Stroke; a Journal of Cerebral Circulation·Midori A YenariRona G Giffard
Mar 4, 2006·Circulation Research·Peter L Hordijk
Aug 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Du-Chu WuSerge Przedborski
Nov 30, 2006·Chembiochem : a European Journal of Chemical Biology·Mario A PaganoLorenzo A Pinna
Jan 24, 2007·Physiological Reviews·Karen Bedard, Karl-Heinz Krause
Jun 23, 2007·Biochimie·Kei Miyano, Hideki Sumimoto
Oct 24, 2007·Molecular and Cellular Biology·David Y LouDavid C Seldin
Feb 5, 2008·Brain Research·Nádia Aléssio VellosoCarlos Fernando Mello
Oct 16, 2008·Biochemical and Biophysical Research Communications·Ikuko KadohiraMasato Yasui
Dec 17, 2008·Current Medicinal Chemistry·Charanjit Kaur, Eng-Ang Ling
Jan 6, 2009·BMC Neuroscience·Michelle L Block
Dec 20, 2008·Mediators of Inflammation·J Stefanska, R Pawliczak
Dec 25, 2008·Annals of Neurology·Sang Won SuhRaymond A Swanson
May 7, 2009·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hai ChenPak H Chan
May 29, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Joo Eun JungPak H Chan

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Citations

Aug 4, 2012·The Journal of Biological Chemistry·Yan ZhangFan Yi
Nov 22, 2012·Brain : a Journal of Neurology·Hiroyuki SakataPak H Chan
Nov 12, 2010·Molecular Neurodegeneration·Young-Don KwakFrancesca-Fang Liao
May 24, 2012·Cellular and Molecular Life Sciences : CMLS·Timo Kahles, Ralf P Brandes
May 16, 2014·The Biochemical Journal·Andrea VenerandoLorenzo A Pinna
Aug 27, 2014·Free Radical Biology & Medicine·Ralf P BrandesKatrin Schröder
Dec 4, 2012·Neurochemistry International·Silvia ManzaneroThiruma V Arumugam
Oct 2, 2013·Developmental Biology·Yogesh P WairkarPhillip Cho
Mar 19, 2014·Antioxidants & Redox Signaling·Angela M Brennan-MinnellaRaymond A Swanson
Dec 8, 2011·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Gab Seok KimPak H Chan
Sep 23, 2010·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hideyuki YoshiokaPak H Chan
Jul 18, 2017·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Anja KahlPing Zhou
Oct 28, 2019·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Carla CirilloIsabelle Loubinoux
Mar 21, 2020·Frontiers in Molecular Neuroscience·Liquan WuLijuan Gu
Jan 18, 2017·Molecular Neurodegeneration·Merry W MaDarrell W Brann
Jun 10, 2017·Molecular and Cellular Biochemistry·Susanne Schaefer, Barbara Guerra
Mar 30, 2021·Oxidative Medicine and Cellular Longevity·Jinnan DuanJifang Sheng
Apr 13, 2021·Critical Reviews in Biochemistry and Molecular Biology·Christian BorgoMauro Salvi

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