Cl- -dependent photovoltage responses of bacteriorhodopsin: comparison of the D85T and D85S mutants and wild-type acid purple form

FEBS Letters
I V Kalaidzidis, A D Kaulen

Abstract

Laser flash-induced photovoltage responses of the D85S and D85T mutants as well as of the wild-type acid blue form are similar and reflect intraprotein charge redistribution caused by retinal isomerization. The Cl- -induced transition of all of these blue forms into purple ones is accompanied by the appearance of electrogenic stages, which is probably associated with Cl- translocation in the cytoplasmic direction. Cl- translocation efficiency of these purple forms is much lower than that of the proton transport by the wild-type bacteriorhodopsin. The values of the efficiency do not exceed 15, 8 and 3% for the D85T, D85S and wild-type acid purple form, respectively. Cl- induces an additional electrogenic phase in the photovoltage responses of the D85S mutant and the wild-type acid purple form. This phase is supposed to be associated with the reversible Cl- movement in the extracellular direction. It is interesting that this component is absent in the photovoltage response of the D85T mutant which has, like halorhodopsin, a threonine residue at position 85.

References

Nov 1, 1979·Biophysical Journal·U Fischer, D Oesterhelt
Jun 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·A DérW Stoeckenius
Jul 24, 1990·Biochemistry·H J de GrootJ Herzfeld
Dec 1, 1989·Biophysical Journal·G Váró, J K Lanyi
Aug 1, 1988·Biophysical Journal·I Szundi, W Stoeckenius
Jul 1, 1981·European Journal of Biochemistry·L A DrachevV P Skulachev
Jul 7, 1995·Science·J SasakiJ K Lanyi

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Citations

Jul 11, 2013·PloS One·Yiyang GongMark J Schnitzer
Nov 2, 2014·Proceedings of the National Academy of Sciences of the United States of America·Yifan Song, M R Gunner
Sep 15, 2004·Trends in Biochemical Sciences·Marc T FacciottiRobert M Glaeser
Mar 10, 2009·Photochemistry and Photobiology·Megumi KuboMakoto Demura
Jul 6, 2015·Current Opinion in Chemical Biology·Yiyang Gong
Nov 27, 2001·Biophysical Journal·G I GromaG Váró
Jun 28, 2003·Biophysical Journal·Marc T FacciottiRobert M Glaeser
Jun 5, 1998·FEBS Letters·I V KalaidzidisA D Kaulen
Apr 28, 2004·FEBS Letters·Marc T FacciottiRobert M Glaeser
Apr 28, 2004·Biochemistry·Marc T FacciottiRobert M Glaeser
Apr 25, 2015·Biochemistry·Sansa DuttaMordechai Sheves
Jun 9, 2018·Chemical Reviews·Christopher EngelhardMartin Engelhard

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