Cl- channels in basolateral renal medullary vesicles. X. Cloning of a Cl- channel from rabbit outer medulla

Kidney International
L ZimniakT E Andreoli

Abstract

These experiments were intended to identify candidate cDNAs which might encode basolateral membrane Cl- channels of the mTAL using a homology-based cloning strategy. We prepared a cDNA library using a 1.8 to 3.2 kb mRNA fraction from rabbit outer medulla that induces a Cl- conductance in cellular membranes of Xenopus laevis oocytes. The cDNA library was screened with two 32P-oligonucleotide probes corresponding to highly conserved sequences in other Cl- channels. We isolated two cDNAs: rbClC-Ka and rbClC-Kb. The protein sequences deduced from these two cDNAs had 99% homology. Using RT-PCR technology, cultured mouse mTAL cells were found to contain mRNA corresponding to those two cDNAs. Expression of the mRNAs corresponding to these two cDNAs was kidney-specific and was greater in rabbit renal medulla than rabbit renal cortex. Finally, by using RT-PCR technology in combination with microdissected glomeruli or tubule segments, we found mRNA for rbClC-Ka in glomeruli, proximal convoluted tubules, mTAL and cortical collecting tubules.

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Citations

Nov 18, 1998·Current Opinion in Nephrology and Hypertension·K Steinmeyer, T J Jentsch
Dec 17, 2003·Kidney International·Nikola JeckSiegfried Waldegger
Feb 22, 2001·Annual Review of Physiology·W B ReevesT E Andreoli
Feb 16, 2005·Annual Review of Physiology·Shinichi Uchida, Sei Sasaki
Jul 23, 2014·PloS One·Lin LiFu-Wu Xing
Jun 1, 2000·The Journal of Biological Chemistry·S Waldegger, T J Jentsch
Jan 22, 1998·The American Journal of Physiology·C J WintersT E Andreoli
Oct 29, 2000·American Journal of Physiology. Renal Physiology·S Uchida
Nov 12, 2002·American Journal of Physiology. Renal Physiology·Olivier Devuyst, William B Guggino
Apr 13, 1999·The American Journal of Physiology·M YoshikawaF Marumo
Jun 23, 2000·Journal of the American Society of Nephrology : JASN·Siegfried Waldegger, Thomas J Jentsch

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