CL22 - a novel cationic peptide for efficient transfection of mammalian cells

Gene Therapy
A M HainesR O Phillips

Abstract

Condensing peptide-DNA complexes have great potential as nonviral agents for gene delivery. To date, however, such complexes have given transfection activities greatly inferior to adenovirus and somewhat inferior to cationic lipid-DNA complexes, even for cell lines and primary cells in vitro. We report here the identification of a novel condensing peptide, CL22, which forms DNA complexes that efficiently transfect many cell lines, as well as primary dendritic and endothelial cells. We report studies with sequence and structure variants that define some properties of the peptide that contribute to efficient transfection. We demonstrate that the superior transfection activity of CL22 compared with other DNA condensing peptides is conferred at a step after uptake of the complexes into cells. We show that CL22-DNA complexes have transfection activity that is at least equivalent to the best available nonviral agents.

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Citations

Dec 20, 2003·Journal of Controlled Release : Official Journal of the Controlled Release Society·Anas El-Aneed
Mar 14, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·W ZaunerA M Haines
Apr 6, 2007·The AAPS Journal·Molly E Martin, Kevin G Rice
Nov 28, 2007·Journal of Materials Science. Materials in Medicine·P PrabuH Y Kim
Aug 24, 2013·Journal of Drug Targeting·Jing YaoLeaf Huang
Jul 30, 2005·Expert Opinion on Biological Therapy·Wilson S Meng, Lisa H Butterfield
Apr 22, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Brenda F CanineArash Hatefi
Apr 4, 2009·Biotechnology Advances·Paolo SaccardoNuria González-Montalbán
Mar 24, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yuhua WangArash Hatefi
Nov 17, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shubiao ZhangZongshi Li
Nov 8, 2014·Clinical and Vaccine Immunology : CVI·Sarah Mackenzie-DyckSylvia van Drunen Littel-van den Hurk
Oct 10, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Gennara CavallaroGaetano Giammona
Aug 22, 2014·BioMed Research International·Suleiman Yusuf AlhajiSyahril Abdullah
Feb 7, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Karola RittnerEric Jacobs
Jul 6, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Kai S LipinskiChristopher J Wrighton
Apr 8, 2003·Cancer Gene Therapy·Gernot RöderPeter Dall
Jul 14, 2007·Journal of Leukocyte Biology·Abdolamir LandiSylvia van Drunen Littel-van den Hurk
Jan 29, 2014·Journal of the Korean Association of Oral and Maxillofacial Surgeons·Jung-Tae LeeTae-Geon Kwon
Jan 11, 2017·Biopolymers·Zachary B JennerKerry A Bruns
Feb 7, 2020·Protein and Peptide Letters·Roya CheraghiSaman Hosseinkhani
May 6, 2003·Biochimica Et Biophysica Acta·Hyun Hee KimSungho Shin
Aug 17, 2021·Frontiers in Chemistry·Patrizia JankovićDaniela Kalafatovic

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