Cladosporium herbarum and Pityrosporum ovale allergen extracts share cross-reacting glycoproteins

International Archives of Allergy and Immunology
Marina LeinoHarri Alenius

Abstract

Patients sensitized to airborne fungi such as Alternaria alternata and Cladosporium herbarum often also show positive skin prick test results and specific serum IgE antibodies to a yeast, Pityrosporum ovale. We examined whether part of the IgE binding to these fungi is explained by cross-reacting mould and yeast allergens. Serum samples from 36 patients with positive skin prick test to A. alternata or C. herbarum were analyzed for IgE antibodies to fungal extracts by ELISA and immunoblot analysis. Cross-reactivity between mould and yeast extracts was studied by ELISA and immunoblot inhibition assays. In further analysis, the mannan-containing glycoproteins were removed from the yeast extract by concanavalin A-Sepharose chromatography, and the IgE binding properties of the extracts were compared. Serum IgE reactivity to P. ovale was found in 40% of the mould-sensitized patients. The IgE antibody binding to A. alternata and C. herbarum moulds was partially inhibited by the yeast P. ovale in ELISA and immunoblot inhibition assays. When the glycoproteins were removed from the extract, cross-reactivity was markedly reduced. Part of the IgE binding to mould and yeast allergen extracts is due to cross-reacting glycoproteins. False-pos...Continue Reading

References

Jan 1, 1992·The Journal of Allergy and Clinical Immunology·E Jensen-JarolimP Stahl Skov
Jan 1, 1988·International Archives of Allergy and Applied Immunology·M Swärd-NordmoJ K Wold
Jan 1, 1997·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·P LintuK Kalimo
May 1, 1997·International Archives of Allergy and Immunology·M BreitenbachR Hirschwehr
Aug 5, 1998·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·J PortnoyC Barnes
Oct 6, 1998·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·D NissenH Nolte
Sep 1, 2000·Microbes and Infection·V P KurupB Banerjee
Jun 26, 2001·Allergy·R C AalberseR van Ree
Apr 30, 2002·International Archives of Allergy and Immunology·Annika ScheyniusMaria Tengvall Linder
Aug 24, 2002·BMJ : British Medical Journal·Mahmoud ZureikUNKNOWN European Community Respiratory Health Survey
Oct 10, 2002·The Journal of Allergy and Clinical Immunology·Harriet A Burge
Oct 10, 2003·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Kari ReijulaTari Haahtela
Feb 21, 2004·International Archives of Allergy and Immunology·Vandana BishtSusheela Sridhara

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Citations

Oct 18, 2006·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Kristiina AirolaSoili Mäkinen-Kiljunen
Aug 23, 2012·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·T E TwarochR Valenta
May 10, 2007·Current Opinion in Allergy and Clinical Immunology

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