Class I Arfs (Arf1 and Arf3) and Arf6 are localized to the Flemming body and play important roles in cytokinesis

Journal of Biochemistry
Ayako HanaiKazuhisa Nakayama

Abstract

Small GTPases play important roles in various aspects of cell division as well as membrane trafficking. We and others previously showed that ADP-ribosylation factor 6 (Arf6) is locally activated around the ingressing cleavage furrow and recruited to the Flemming body in late cytokinesis phases, and involved in faithful completion of cytokinesis. However, knockout of the Arf6 gene or Arf6 depletion by siRNAs did not drastically influence cytokinesis. We here show that, in addition to Arf6, Class I Arfs (Arf1 and Arf3) are localized to the Flemming body, and that double knockdown of Arf1 and Arf3 moderately increases the proportion of multinucleate cells and simultaneous knockdown of Arf1, Arf3 and Arf6 leads to severe cytokinesis defects. These observations indicate that Arf1 and Arf3 as well as Arf6 play important roles in cytokinesis. We further show that EFA6 (exchange factor for Arf6) activates not only Arf6 but also Arf1 in the cell. Taken together with our previous data, these Arf GTPases are likely to be locally activated by EFA6 and in turn targeted to the Flemming body to complete cytokinesis.

References

May 23, 2002·The Journal of Biological Chemistry·Jill Kuglin Schweitzer, Crislyn D'Souza-Schorey
Jan 27, 2005·Journal of Biochemistry·Hye-Won Shin, Kazuhisa Nakayama
Mar 19, 2005·Science·Michael Glotzer
Jul 13, 2005·Trends in Cell Biology·Marisa S OteguiAhna R Skop
Sep 27, 2005·Experimental Cell Research·Jill Kuglin Schweitzer, Crislyn D'Souza-Schorey
Jan 18, 2006·Methods in Enzymology·Hye-Won ShinKazuhisa Nakayama
Mar 1, 2006·The Journal of Cell Biology·Richard A KahnAnnette Schurmann
Apr 25, 2006·Nature Reviews. Molecular Cell Biology·Crislyn D'Souza-Schorey, Philippe Chavrier
May 18, 2007·Annual Review of Cell and Developmental Biology·Alison K Gillingham, Sean Munro
Mar 8, 2008·The Tohoku Journal of Experimental Medicine·Hiroyuki Sakagami
Mar 11, 2008·Trends in Cell Biology·Kenneth R Myers, James E Casanova
May 13, 2008·Current Opinion in Cell Biology·Guillaume MontagnacPhilippe Chavrier
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Eiji MoritaWesley I Sundquist
Aug 25, 2010·Seminars in Cell & Developmental Biology·Max E Douglas, Masanori Mishima
Nov 12, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Masahiro AkiyamaYasunori Kanaho
May 19, 2011·Nature Reviews. Molecular Cell Biology·Julie G Donaldson, Catherine L Jackson
Jul 28, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Senye TakahashiKazuhisa Nakayama
Dec 22, 2011·Cell Structure and Function·Kouhei TakashimaKazuhisa Nakayama
Jan 27, 2012·Molecular Biology of the Cell·Chi-Kuo HuTimothy J Mitchison
May 15, 2012·The EMBO Journal·Nimesh JosephMasanori Mishima
Sep 14, 2012·Cell Structure and Function·Yumika KondoHye-Won Shin
Nov 28, 2012·Current Opinion in Cell Biology·John A Schiel, Rytis Prekeris
Jan 19, 2013·Cell Structure and Function·Hiroyuki TakatsuKazuhisa Nakayama

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Citations

Jan 25, 2019·Development·Mariko Kinoshita-KawadaJane Y Wu
Mar 2, 2018·Annual Review of Biochemistry·Elisabeth M StorckUlrike S Eggert
Aug 22, 2020·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Haiming LeiBo Tan
May 16, 2019·Molecular Biology of the Cell·Elizabeth SztulRichard A Kahn
Sep 10, 2020·Journal of Bacteriology·Alfa Herrera, Karla J F Satchell

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