PMID: 7539044Jun 1, 1995Paper

Class I major histocompatibility complex-restricted cytotoxic T lymphocytes specific for Epstein-Barr virus (EBV)-transformed B lymphoblastoid cell lines against which they were raised

The Journal of Experimental Medicine
A B HillA J McMichael

Abstract

We have raised CD8+ cytotoxic T lymphocytes (CTL) from three Epstein-Barr virus-seropositive donors by incubating peripheral blood lymphocytes with irradiated autologous B95.8-strain EBV-transformed B lymphoblastoid cells (LCL). However, to detect lysis in a standard 51Cr release assay of the LCL against which these CTL were raised, superinfection with recombinant vaccinia expressing the appropriate EBV protein or incubation with the peptide epitope was necessary. The untreated LCL were not lysed, even though Western blotting demonstrated that they expressed the EBV antigens containing the CTL epitopes. We have found CTL of this phenotype that are restricted by human leukocyte antigen-A2, -A3, -B7, or -B39, and which recognize the EBV latent proteins, EBV nuclear antigen (EBNA)-3A, EBNA-3C, or terminal protein. During these experiments, we identified a new human leukocyte antigen-A3-restricted EBNA-3A epitope, residues 603-611, RLRAEAGVK. We raised a spontaneous LCL, transformed by endogenous EBV, from one donor, but this was also not lysed. For at least one of the epitopes, CTL from another donor lysed the LCL without superinfection or addition of peptides. We conclude that the CTL were unable to achieve a high enough avidity ...Continue Reading

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Citations

Sep 19, 2002·International Journal of Cancer. Journal International Du Cancer·Riccardo GavioliMaria G Masucci
Aug 1, 1996·Current Opinion in Immunology·A B RickinsonN M Steven
May 3, 2003·Trends in Molecular Medicine·Stanislav VukmanovićFabio R Santori
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·R KhannaD J Moss
May 19, 2004·The Journal of Experimental Medicine·Steven P LeeNeil W Blake
May 18, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Nicola E AnnelsArjan C Lankester
Nov 19, 2008·Journal of Neurology, Neurosurgery, and Psychiatry·M P PenderS R Burrows
Jan 1, 1997·Annual Review of Immunology·A B Rickinson, D J Moss
Jun 8, 1999·Annual Review of Immunology·J W Yewdell, J R Bennink
Mar 10, 2011·Annals of Neurology·Matthias MehlingChristoph Hess
Aug 30, 2000·European Journal of Clinical Investigation·W KampH S Nottet
Nov 10, 2006·Journal of Molecular Recognition : JMR·Gokhan DevirenJonathan P Schneck
Feb 10, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jill M BrooksAlan B Rickinson
Mar 31, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Samantha JilekRenaud A Du Pasquier
Jun 22, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S P LeeD P Huang
Feb 13, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·C OhlénP D Greenberg
Oct 10, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M D CatalinaK Luzuriaga
May 8, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Laura SantodonatoMaria Ferrantini

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