Class III peroxidases in cellulose deficient cultured maize cells during cell wall remodeling

Physiologia Plantarum
Romina Martínez-RubioAnna Kärkönen

Abstract

Maize (Zea mays L.) suspension-cultured cells habituated to a cellulose biosynthesis inhibitor 2,6-dichlorobenzonitrile (DCB) have a modified cell wall, in which the reduction in the cellulose content is compensated by a network of highly cross-linked feruloylated arabinoxylans and the deposition of lignin-like polymers. For both arabinoxylan cross-linking and lignin polymerization, class III peroxidases (POXs) have been demonstrated to have a prominent role. For the first time, a comparative study of POX activity and isoforms in control and cellulose-impaired cells has been addressed, also taking into account their cellular distribution in different compartments. Proteins from the spent medium (SM), soluble cellular (SC), ionically (ICW) and covalently bound cell wall protein fractions were assayed for total and specific peroxidase activity by using coniferyl and sinapyl alcohol and ferulic acid as substrates. The isoPOX profile was obtained by isoelectric focusing. POX activity was higher in DCB-habituated than in non-habituated cells in all protein fractions at all cell culture stages. For all substrates assayed, SC and ICW fractions showed higher activity at the early log growth phase than at the late log phase. However, th...Continue Reading

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Citations

Jun 30, 2018·Physiologia Plantarum·Thorsten HamannKirsten Krause
Nov 22, 2020·International Journal of Molecular Sciences·Mirza HasanuzzamanMasayuki Fujita
Nov 27, 2020·International Journal of Molecular Sciences·Anne HofmannSabine Lüthje

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