Class VIII myosins are required for plasmodesmatal localization of a closterovirus Hsp70 homolog

Journal of Virology
Dror AvisarV V Dolja

Abstract

The Hsp70 homolog (Hsp70h) of Beet yellows virus (BYV) functions in virion assembly and cell-to-cell movement and is autonomously targeted to plasmodesmata in association with the actomyosin motility system (A. I. Prokhnevsky, V. V. Peremyslov, and V. V. Dolja, J. Virol. 79:14421-14428, 2005). Myosins are a diverse category of molecular motors that possess a motor domain and a tail domain involved in cargo binding. Plants have two classes of myosins, VIII and XI, whose specific functions are poorly understood. We used dominant negative inhibition to identify myosins required for Hsp70h localization to plasmodesmata. Six full-length myosin cDNAs from the BYV host plant Nicotiana benthamiana were sequenced and shown to encode apparent orthologs of the Arabidopsis thaliana myosins VIII-1, VIII-2, VIII-B, XI-2, XI-F, and XI-K. We found that the ectopic expression of the tail domains of each of the class VIII, but not the class XI, myosins inhibited the plasmodesmatal localization of Hsp70h. In contrast, the overexpression of the motor domains or the entire molecules of the class VIII myosins did not affect Hsp70h targeting. Further mapping revealed that the minimal cargo-binding part of the myosin VIII tails was both essential and ...Continue Reading

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Citations

Oct 13, 2010·Protoplasma·Jens TilsnerLesley Torrance
Dec 3, 2010·Protoplasma·Annette Niehl, Manfred Heinlein
Dec 1, 2010·Protoplasma·Raul ZavalievVitaly Citovsky
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Jun 1, 2018·Annual Review of Phytopathology·Scott M Leisner, James E Schoelz

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