Classical and membrane-initiated estrogen signaling in an in vitro model of anterior hypothalamic kisspeptin neurons

Endocrinology
Melinda A Mittelman-SmithPaul Micevych

Abstract

The neuropeptide kisspeptin is essential for sexual maturation and reproductive function. In particular, kisspeptin-expressing neurons in the anterior rostral periventricular area of the third ventricle are generally recognized as mediators of estrogen positive feedback for the surge release of LH, which stimulates ovulation. Estradiol induces kisspeptin expression in the neurons of the rostral periventricular area of the third ventricle but suppresses kisspeptin expression in neurons of the arcuate nucleus that regulate estrogen-negative feedback. To focus on the intracellular signaling and response to estradiol underlying positive feedback, we used mHypoA51 cells, an immortalized line of kisspeptin neurons derived from adult female mouse hypothalamus. mHypoA51 neurons express estrogen receptor (ER)-α, classical progesterone receptor (PR), and kisspeptin, all key elements of estrogen-positive feedback. As with kisspeptin neurons in vivo, 17β-estradiol (E2) induced kisspeptin and PR in mHypoA51s. The ERα agonist, 1,3,5-Tris(4-hydroxyphenyl)-4-propyl-1H-pyrazole, produced similar increases in expression, indicating that these events were mediated by ERα. However, E2-induced PR up-regulation required an intracellular ER, whereas ...Continue Reading

References

Oct 6, 2001·Frontiers in Neuroendocrinology·A E Herbison, J R Pape
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Aug 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Nicolas de RouxEdwin Milgrom
Oct 24, 2003·The New England Journal of Medicine·Stephanie B SeminaraWilliam H Colledge
Apr 16, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Larry N PetzAnn M Nardulli
May 20, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Marissa I BoulwarePaul G Mermelstein
May 28, 2005·Endocrinology·Jeremy T SmithRobert A Steiner
Aug 3, 2005·BioTechniques·Marisa L Wong, Juan F Medrano
Dec 13, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Seong-Kyu HanAllan E Herbison
Nov 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Shuibing ChenSheng Ding
Mar 23, 2007·Reviews in Endocrine & Metabolic Disorders·Kei-Ichiro MaedaHiroko Tsukamura
May 2, 2007·Proceedings of the National Academy of Sciences of the United States of America·C Glidewell-KenneyJ L Jameson
May 10, 2007·The Journal of Clinical Endocrinology and Metabolism·Adonna M RometoNaomi E Rance
May 31, 2007·The Journal of Biological Chemistry·Ali PedramEllis R Levin
Jul 20, 2007·Reproductive Sciences·Gemma MadsenRoger Smith
Jan 15, 2008·Results and Problems in Cell Differentiation·W H Colledge
Jun 24, 2008·Molecular and Cellular Endocrinology·Paul Micevych, Kevin Sinchak
Aug 30, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jenny ClarksonAllan E Herbison
Apr 25, 2009·Molecular Pharmacology·Ester Gutiérrez-PascualJusto P Castaño
Jul 25, 2009·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Michelle L GottschRobert A Steiner
Aug 29, 2009·The Journal of Biological Chemistry·Kee K KimSachiyo Kawamoto
Sep 11, 2009·Trends in Endocrinology and Metabolism : TEM·Lazar Z KrsmanovicKevin J Catt

❮ Previous
Next ❯

Citations

Nov 1, 2017·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Charlotte A Cornil
Aug 22, 2015·Endocrinology·Kimberly H Cox
Jun 23, 2016·Journal of Neuroendocrinology·L M RudolphP E Micevych
Aug 10, 2017·Frontiers in Endocrinology·Melinda A Mittelman-SmithPaul E Micevych
Dec 31, 2015·Human Reproduction Update·Roberto MaggiPatrizia Limonta
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Si-Si ZhangZhi-Yang Zhou
Jun 22, 2016·Molecular Neurobiology·Yu-Jie LaiGuo-Jun Chen

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.