PMID: 15227788Jul 2, 2004Paper

Classical and molecular approaches as a powerful tool for the characterization of rumen polycentric fungi

Folia Microbiologica
K FliegerováKerstin Voigt

Abstract

Ribosomal ITS1 and ITS2 fragments from 8 isolates of polycentric rumen anaerobic fungi were PCR-amplified and sequenced; the sequences obtained were aligned with published data and phylogenetic analyses were performed. Analysis of the ITS1 fragment clearly differentiated between the two polycentric genera Orpinomyces and Anaeromyces and this classification is supported by morphological observation. A multi-order phylogram based on ITS2 sequences proved that anaerobic rumen fungi are separated from aerobic chytrids, which form a well-supported monophylum with the highest possible bootstrap proportion values of 100%. Sequence analysis of ITS regions is a powerful tool for classification of anaerobic fungi but morphological description of strains is still necessary because some genera of rumen fungi display a high genetic heterogeneity.

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Citations

Sep 30, 2006·Folia Microbiologica·K FliegerováK Voigt
Jul 12, 2012·Applied Microbiology and Biotechnology·Sunil Kumar SirohiAnil Kumar Puniya
Jul 29, 2008·Folia Microbiologica·I KoppováK Fliegerová
Aug 4, 2010·Folia Microbiologica·Z NovotnáK Fliegerová
Jul 26, 2011·Applied and Environmental Microbiology·Sumit S DagarAnil K Puniya
Oct 14, 2016·Antonie van Leeuwenhoek·Xuewei WangJohannes Z Groenewald

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