Classification and characterization of the rice alpha-amylase multigene family

Plant Molecular Biology
N HuangR L Rodriguez

Abstract

To establish the size and organization of the rice alpha-amylase multigene family, we have isolated 30 alpha-amylase clones from three independent genomic libraries. Partial characterization of these clones indicates that they fall into 5 hybridization groups containing a total of 10 genes. Two clones belonging to the Group 3 hybridization class have more than one gene per cloned fragment. The nucleotide sequence of one clone from Group 1, lambda OSg2, was determined and compared to other known cereal alpha-amylase sequences revealing that lambda OSg2 is the genomic analog of the rice cDNA clone, pOS103. The rice alpha-amylase genes in Group 1 are analogous to the alpha-Amy1 genes in barley and wheat. lambda OSg2 contains sequence motifs common to most actively transcribed genes in plants. Two consensus sequences, TAACAAGA and TATCCAT, were found in the 5' flanking regions of alpha-amylase genes of rice, barley and wheat. The former sequence may be specific to alpha-amylase gene while the latter sequence may be related to a 'CATC' box found in many plant genes. Another sequence called the pyrimidine box (TCCTTTTTC) was found in the alpha-amylase genes as well as other genes regulated by gibberellic acid (GA). Comparisons based ...Continue Reading

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Citations

Aug 20, 1991·Biotechnology and Bioengineering·C R SimmonsR L Rodriguez
May 1, 1993·Plant Molecular Biology·D E MalehornD M Shah
May 1, 1991·Plant Molecular Biology·E E KarrerR L Rodriguez
Dec 1, 1994·Plant Molecular Biology·R Hooley
Apr 1, 1991·Plant Molecular Biology·T D SutliffR L Rodriguez
Mar 1, 1991·Plant Molecular Biology·B GopalakrishnanS Muthukrishnan
Dec 1, 1992·Plant Molecular Biology·F J CejudoD C Baulcombe
May 1, 1992·Plant Molecular Biology·J C LittsR L Rodriguez
Jan 1, 1992·Plant Molecular Biology·J K Kim, R Wu
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Dec 11, 1990·Nucleic Acids Research·N HuangR L Rodriguez
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