Classification and risk stratification of invasive breast carcinomas using a real-time quantitative RT-PCR assay.

Breast Cancer Research : BCR
Laurent PerreardPhilip S Bernard

Abstract

Predicting the clinical course of breast cancer is often difficult because it is a diverse disease comprised of many biological subtypes. Gene expression profiling by microarray analysis has identified breast cancer signatures that are important for prognosis and treatment. In the current article, we use microarray analysis and a real-time quantitative reverse-transcription (qRT)-PCR assay to risk-stratify breast cancers based on biological 'intrinsic' subtypes and proliferation. Gene sets were selected from microarray data to assess proliferation and to classify breast cancers into four different molecular subtypes, designated Luminal, Normal-like, HER2+/ER-, and Basal-like. One-hundred and twenty-three breast samples (117 invasive carcinomas, one fibroadenoma and five normal tissues) and three breast cancer cell lines were prospectively analyzed using a microarray (Agilent) and a qRT-PCR assay comprised of 53 genes. Biological subtypes were assigned from the microarray and qRT-PCR data by hierarchical clustering. A proliferation signature was used as a single meta-gene (log2 average of 14 genes) to predict outcome within the context of estrogen receptor status and biological 'intrinsic' subtype. We found that the qRT-PCR assa...Continue Reading

References

Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M B EisenD Botstein
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·C M PerouD Botstein
Aug 30, 2000·Nature·C M PerouD Botstein
Jun 8, 2001·Bioinformatics·O TroyanskayaR B Altman
Sep 13, 2001·Proceedings of the National Academy of Sciences of the United States of America·T SørlieA L Børresen-Dale
Feb 2, 2002·Nature·Laura J van 't VeerStephen H Friend
Jun 12, 2002·Molecular Biology of the Cell·Michael L WhitfieldDavid Botstein
Aug 20, 2002·Genome Biology·Sandrine Dudoit, Jane Fridlyand
Dec 20, 2002·The New England Journal of Medicine·Marc J van de VijverRené Bernards
Jan 10, 2003·Nucleic Acids Research·Maximilian DiehnAsh A Alizadeh
Feb 20, 2003·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Michael BilousMarc van de Vijver
Jun 28, 2003·Proceedings of the National Academy of Sciences of the United States of America·Therese SorlieDavid Botstein
Aug 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christos SotiriouEdison T Liu
Dec 25, 2003·Bioinformatics·Monica BenitoJ S Marron
Apr 16, 2004·The New England Journal of Medicine·Lars BullingerJonathan R Pollack
Apr 20, 2004·PLoS Biology·Eric Bair, Robert Tibshirani
Aug 4, 2004·Genome Biology·Aniko SzaboPhilip S Bernard
Aug 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Kun YuPatrick Tan
Dec 14, 2004·The New England Journal of Medicine·Soonmyung PaikNorman Wolmark
Feb 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Howard Y ChangMarc J van de Vijver
Feb 16, 2006·Journal of the National Cancer Institute·Christos SotiriouMauro Delorenzi

❮ Previous
Next ❯

Citations

Feb 22, 2012·Cancer Chemotherapy and Pharmacology·Che LinHwei-Chung Wang
Dec 4, 2012·Breast Cancer Research and Treatment·Jorma J de RondeLodewyk F A Wessels
Aug 6, 2013·Breast Cancer Research and Treatment·Julia Y S TsangGary M Tse
Mar 25, 2009·Clinical & Experimental Metastasis·Ulrich PfefferAdriana Albini
Mar 4, 2008·Breast Cancer : the Journal of the Japanese Breast Cancer Society·Shunzo Kobayashi
Jan 15, 2009·Nature Reviews. Cancer·Erik S Knudsen, Karen E Knudsen
Oct 10, 2012·Nature Reviews. Genetics·Lucy R Yates, Peter J Campbell
Nov 21, 2012·Annals of Oncology : Official Journal of the European Society for Medical Oncology·S GuiuJ S Reis-Filho
Jan 31, 2012·Japanese Journal of Clinical Oncology·Che LinDar-Ren Chen
Mar 8, 2008·Pathology International·Minseob EomKwang Hwa Park
Apr 8, 2010·Science Translational Medicine·Serge Koscielny
Sep 3, 2009·Cancer Research·Aedín C Culhane, John Quackenbush
Feb 4, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Andrew H BeckRobert B West
Apr 23, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Aurélie DocquierVincent Cavaillès
Jun 20, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Stephen K ChiaTorsten O Nielsen
Nov 15, 2007·Cytogenetic and Genome Research·V Urquidi, S Goodison
Jun 17, 2011·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·Anthony S-Y Leong, Zhengping Zhuang
Oct 4, 2011·The Journal of Clinical Investigation·Nicholas R Bertos, Morag Park
Aug 1, 2007·BMC Genomics·Katherine A HoadleyCharles M Perou
May 24, 2012·Journal of Clinical Bioinformatics·Xiaowei GuanJill S Barnholtz-Sloan
Feb 1, 2011·Breast Cancer Research : BCR·Lisa M ArendtLinda A Schuler
Feb 11, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Joel S ParkerPhilip S Bernard
Sep 24, 2010·Molecular Endocrinology·Daniel J Toft, Vincent L Cryns
Oct 24, 2009·PloS One·Haleh YasrebiPhilipp Bucher
Jul 31, 2013·PloS One·Yesim Gökmen-PolarSunil Badve
Dec 1, 2007·Biomarkers in Medicine·Andrea NicoliniPaolo Miccoli
Feb 18, 2012·Future Oncology·Andrea NicoliniAlessandro Antonelli
Apr 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alana L WelmJ Michael Bishop

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE2607

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST
R Foundation for Statistical Computing
GenePattern
GenePix Pro
Lightcycler
Lightcycler Probe Design
Evidence viewer
R
BLAT
Cluster

Related Concepts

Related Feeds

Cancer Incidence & Mortality

Cancer has emerged as a global concern due to its increase in incidence and mortality. Efforts are underway to evaluate and develop action plans to reduce the global burden of cancer. Currently, lung cancer, breast cancer and prostate cancer are the leading causes of cancer mortality. Here is the latest research on cancer incidence and mortality.

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Cancer Genomics (Keystone)

Cancer genomics approaches employ high-throughput technologies to identify the complete catalog of somatic alterations that characterize the genome, transcriptome and epigenome of cohorts of tumor samples. Discover the latest research using such technologies in this feed.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Cancer: Chemo-Resistance

Some cancers are difficult to treat and aggressive including the "triple-negative" breast cancer. This type of cancer is chemoresistant even before chemotherapy begins. Here are the latest discoveries chemo-resistance in breast cancer.