Apr 25, 2015

Classification of circulating tumor cells by epithelial-mesenchymal transition markers

PloS One
Shiyang WuJiasen Xu

Abstract

In cancer, epithelial-mesenchymal transition (EMT) is associated with metastasis. Characterizing EMT phenotypes in circulating tumor cells (CTCs) has been challenging because epithelial marker-based methods have typically been used for the isolation and detection of CTCs from blood samples. The aim of this study was to use the optimized CanPatrol CTC enrichment technique to classify CTCs using EMT markers in different types of cancers. The first step of this technique was to isolate CTCs via a filter-based method; then, an RNA in situ hybridization (RNA-ISH) method based on the branched DNA signal amplification technology was used to classify the CTCs according to EMT markers. Our results indicated that the efficiency of tumor cell recovery with this technique was at least 80%. When compared with the non-optimized method, the new method was more sensitive and more CTCs were detected in the 5-ml blood samples. To further validate the new method, 164 blood samples from patients with liver, nasopharyngeal, breast, colon, gastric cancer, or non-small-cell lung cancer (NSCLC) were collected for CTC isolation and characterization. CTCs were detected in 107 (65%) of 164 blood samples, and three CTC subpopulations were identified using...Continue Reading

Mentioned in this Paper

Keratin-19
Pathologic Cytolysis
Biological Markers
Cancer of Nasopharynx
Study
Tumor Cells, Uncertain Whether Benign or Malignant
KRT19 wt Allele
Nasopharyngeal Carcinoma
VIM gene
Classification

Related Feeds

Breast Invasive Carcinoma (Keystone)

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Breast Invasive Carcinoma

Invasive breast cancers indicate a spread into breast tissues and lymph nodes. Here are the latest discoveries pertaining to breast invasive carcinomas.

Related Papers

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Simon A JoosseKlaus Pantel
Recent Results in Cancer Research. Fortschritte Der Krebsforschung. Progrès Dans Les Recherches Sur Le Cancer
Maryam LustbergJeffrey J Chalmers
Advances in Clinical Chemistry
Eunice López-Muñoz, Manuel Méndez-Montes
© 2020 Meta ULC. All rights reserved