Classification of fungal glucuronoyl esterases (FGEs) and characterization of two new FGEs from Ceriporiopsis subvermispora and Pleurotus eryngii

Applied Microbiology and Biotechnology
Meng-I LinMasato Katahira

Abstract

Fungal glucuronoyl esterases (FGEs) catalyze cleavage of the ester bond connecting a lignin alcohol to the xylan-bound 4-O-methyl-D-glucuronic acid of glucuronoxylans. Thus, FGEs are capable of degrading lignin-carbohydrate complexes and have potential for biotechnological applications toward woody biomass utilization. Therefore, identification and characterization of new FGEs are of critical importance. Firstly, in this study, we built a phylogenetic tree from almost 400 putative FGEs obtained on BLAST analysis and defined six main clades. In the phylogenetic tree, all the putative FGEs of ascomycetes cluster in clades I to IV, and most of the putative FGEs of basidiomycetes (B-FGEs) cluster in clades V to VI. Interestingly, several B-FGEs were found to cluster in clade II; most FGEs of clade II were found to have higher theoretical isoelectric points than those in the other five clades. To gain an insight into the putative FGEs in the clades that have not been characterized yet, we chose the FGEs of Ceriporiopsis subvermispora (CsGE) and Pleurotus eryngii (PeGE), which belong to clades V and II, respectively. The catalytic domains of both CsGE and PeGE were successfully expressed using Pichia pastoris, and then purified. Benz...Continue Reading

References

Jun 1, 1992·Computer Applications in the Biosciences : CABIOS·D T JonesJ M Thornton
Aug 1, 2006·FEBS Letters·Silvia Spániková, Peter Biely
Apr 19, 2007·Archives of Microbiology·Silvia SpánikováPeter Biely
Aug 7, 2007·FEBS Letters·Xin-Liang LiPeter Biely
Oct 16, 2008·Archives of Microbiology·Miroslava DuranováRonald P de Vries
Oct 13, 2009·Biochemistry·Vincent VagenendeBernhardt L Trout
Nov 10, 2009·Bioscience, Biotechnology, and Biochemistry·Miroslava DuranováPeter Biely
May 18, 2010·Applied Microbiology and Biotechnology·Evangelos TopakasPaul Christakopoulos
Jun 11, 2011·Proteins·Phani Raj PokkuluriMarianne Schiffer
Oct 1, 2011·Nature Methods·Thomas Nordahl PetersenHenrik Nielsen
May 31, 2012·Nucleic Acids Research·Yanbin YinYing Xu
Jan 1, 2013·Acta Crystallographica. Section D, Biological Crystallography·Maria Despoina CharavgiEvangelia D Chrysina
Sep 26, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hampus SunnerPaul Christakopoulos
Dec 30, 2015·Journal of Biotechnology·Clotilde d'ErricoRune Nygaard Monrad
Sep 9, 2016·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Chunliang XieYuande Peng
Mar 16, 2017·Frontiers in Microbiology·Jane W AggerLene Lange
Apr 22, 2017·Applied Microbiology and Biotechnology·Silvia HüttnerLisbeth Olsson

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Citations

Apr 6, 2019·Applied Microbiology and Biotechnology·Caroline MosbechJane Wittrup Agger

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Datasets Mentioned

BETA
MH361011
MH361012

Methods Mentioned

BETA
PCR

Software Mentioned

ExPASy ProtParam tool
BLASTP
SignalP
ExPASy ProtParam
ClustalW
BLAST
MEGA7
Graph Pad Prism
dbCAN

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