Classification of human adenoviruses by SDS-polyacrylamide gel electrophoresis of structural polypeptides

Intervirology
G Wadell

Abstract

The virion polypeptide compositions of 15 human adenovirus types, representing all members of subgroups B and C and selected members of subgroups A and D, have been analyzed by SDS-PAGE. The capsid polypeptides II, III, IIIa and IV displayed a pattern of apparent molecular weights which was characteristic for each adenovirus serotype analyzed. Adenovirus serotypes belonging to the same subgroup were also demonstrated to share an apparent molecular weight pattern of the internal structural polypeptides in the following manner: Subgroup A:V-resolved into two bands of 46--48.5 K and 51--51.5 K; VI-25.5 K; and VII-18 K. Subgroup B:V-53.5--54.5 K; VI-24 K; and VII-18 K. Subgroup C:V-48.5 K; VI-24 K; and VII-18.5 K. Subgroup D:V-50.5 K; VI-23.5 K; and VII-18 K. Adenovirus type 4: V-48 K; VI-24.5 K; and VII-18 K. The structural polypeptides represent a major portion of the adenovirus gene products. It is therefore proposed that the apparent molecular weight differences of viral polypeptides be used (i) to confirm the identification of an adenovirus type, and (ii) for subgroup classification of adenoviruses and, consequently, that adenovirus type 4 should be classified as a member of a separate subgroup designated E.

Citations

Mar 1, 1981·Biulleten' eksperimental'noĭ biologii i meditsiny·I S KhromovE E Zolotarskaia
Jan 1, 1986·Archives of Virology·M J Albert
Jan 1, 1979·Journal of Immunological Methods·M E JohanssonL Svensson
Jul 1, 1986·Journal of Virological Methods·J Weber, H A Ménard
Jun 1, 1989·Journal of Virological Methods·J D Connor, P Walpita
Apr 10, 2007·The Journal of Surgical Research·Willem A MarsmanJ Jan B van Lanschot
Jul 1, 1987·Analytical Biochemistry·J F CupoJ A Harpst
Jul 1, 1982·The Journal of Pediatrics·R H YolkenS E Strauss
Jan 1, 1992·Revista Do Instituto De Medicina Tropical De São Paulo·T M de PaivaJ Kalil
Nov 13, 2004·Antiviral Chemistry & Chemotherapy·Kunikazu KurosakiKimiyasu Shiraki
Jan 1, 1980·Annals of the New York Academy of Sciences·G WadellG Sundell
Jan 1, 1980·Annals of the New York Academy of Sciences·J SambrookT R Broker
Apr 1, 2005·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Marta BakayTibor Olah
Sep 1, 1984·Journal of Clinical Microbiology·I UhnooM E Johansson
Feb 1, 1981·Journal of Clinical Microbiology·J C Hierholzer, F H Rodriguez
Jul 1, 1986·Journal of Clinical Microbiology·A S Bailey, S J Richmond
May 1, 1981·Infection and Immunity·G GernaG Achilli
Feb 1, 1986·Journal of Clinical Microbiology·F R BishaiE Rossier

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