Classification of malignant gliomas by infrared spectroscopy and linear discriminant analysis

Biopolymers
Christoph KrafftReiner Salzer

Abstract

Gliomas are the most frequent primary brain tumors. Their malignancies are graded from 1 to 4. Malignant gliomas are astrocytoma grade 3 and glioblastoma grade 4. An IR spectroscopic approach is presented to diagnose brain tissue at the molecular level probing chemical and structural properties without external markers. IR spectroscopic maps were recorded in transmission mode by sequential acquisition of IR spectra. Training spectra of various tissue types are selected from IR spectroscopic maps in accordance with histological assessment of hematoxylin and eosin stained parallel tissue sections. A decrease of the lipid-to-protein ratio in IR spectra is correlated with the malignancy of gliomas. This chemical property is described by the band intensity ratio 2850 to 1655 cm(-1). Two additional molecular descriptors are identified at 1545 cm(-1)/1655 cm(-1) and (1231 + 1450) cm(-1)/1655 cm(-1), which are associated with hemoglobin and collagen, respectively. This metric is used to train a classification model based on linear discriminant analysis. The model is applied to classify normal brain tissue, astrocytoma grade 2, astrocytoma grade 3, glioblastoma, hemorrhage, and leptomeninges in IR spectroscopic maps of cryosections from...Continue Reading

Citations

Nov 15, 2006·Analytical and Bioanalytical Chemistry·Christoph KrafftReiner Salzer
Jan 6, 2007·Analytical and Bioanalytical Chemistry·Venkata Radhakrishna KondepatiJuergen Backhaus
Jul 20, 2007·Analytical and Bioanalytical Chemistry·Christoph KrafftReiner Salzer
Jan 30, 2008·Analytical and Bioanalytical Chemistry·Claudia Beleites, Reiner Salzer
Apr 2, 2008·Analytical and Bioanalytical Chemistry·Liqun Wang, Boris Mizaikoff
Oct 17, 2008·Analytical and Bioanalytical Chemistry·Stephan B SobottkaChristoph Krafft
Mar 4, 2011·The Analyst·Li-Lin TayMahmud Bani-Yaghoub
Jan 15, 2015·Neurosurgical Focus·Imad Saeed Khan, Moneeb Ehtesham
Mar 3, 2011·Journal of Biomedical Optics·Tobias MeyerJürgen Popp
Nov 5, 2008·Journal of Biomedical Materials Research. Part a·Yong WangRanganathan Parthasarathy
Sep 1, 2007·Biochimica Et Biophysica Acta·Nadia AmharrefMichel Manfait
Apr 10, 2012·Biotechnology Advances·Razia NoreenCyril Petibois
Mar 7, 2014·International Journal of Radiation Biology·Ortrud UckermannMatthias Kirsch
Jan 16, 2013·Analytical and Bioanalytical Chemistry·Li-Fang ChiuDar-Bin Shieh
Dec 21, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ortrud UckermannMatthias Kirsch
Dec 6, 2019·Biomedical Physics & Engineering Express·Tammar Hussein Ali, Ammar Alhasan
Feb 17, 2021·Analytical Chemistry·Warda BoutegrabetCyril Gobinet
Jun 24, 2021·Journal of Biophotonics·Ella WillenbacherJohannes D Pallua
Nov 24, 2021·Obesity Surgery·Małgorzata WrzosekAgnieszka Białek

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.