Classifying bivalve larvae using shell pigments identified by Raman spectroscopy

Analytical and Bioanalytical Chemistry
Christine M ThompsonSheri N White

Abstract

Because bivalve larvae are difficult to identify using morphology alone, the use of Raman spectra to distinguish species could aid classification of larvae collected from the field. Raman spectra from shells of bivalve larvae exhibit bands that correspond to polyene pigments. This study determined if the types of shell pigments observed in different species could be unique enough to differentiate larvae using chemotaxonomic methods and cluster analysis. We collected Raman spectra at three wavelengths from 25 samples of bivalve larvae representing 16 species and four taxonomic orders. Grouping spectra within general categories based on order/family relationships successfully classified larvae with cross-validation accuracies ≥92% for at least one wavelength or for all wavelengths combined. Classifications to species were more difficult, but cross-validation accuracies above 86% were observed for 7 out of 14 species when tested using species groups within orders/families at 785 nm. The accuracy of the approach likely depends on the composition of species in a sample and the species of interest. For example, high classification accuracies (85-98%) for distinguishing spectra from Crassostrea virginica larvae were achieved with a se...Continue Reading

References

Aug 12, 2003·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·H G M EdwardsR I Lewis-Smith
Jul 31, 2004·Analytical Chemistry·Wei E HuangAndrew S Whiteley
Mar 23, 2007·Analytical and Bioanalytical Chemistry·Kris De GussemLuc Moens

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