Clathrin and clathrin-accessory proteins in rat kidney cortex epithelia.

Histochemistry and Cell Biology
Sabine HasseStephanie Groos

Abstract

Several vectorial transport routes in mammalian cells involve clathrin and associated proteins. In kidney epithelia urine production requires numerous transport processes. However, only little is known about the distribution of clathrin and its associated proteins in this organ in situ. We now report on the presence and distribution of clathrin and its accessory proteins AP1, AP2, Eps15, Epsin, CALM and Clint/EpsinR in the epithelia of the rat kidney cortex using immunoblotting, immunofluorescence and immuno-electron microscopy. Our data show that all investigated proteins are ubiquitously present in rat kidney cortex epithelia, however, with distinct distribution patterns. In the renal corpuscle, podocytes showed the most conspicuous labelling. Clathrin, AP2 and CALM were highly expressed in foot processes, while AP1 was primarily localized in the cell body. In the proximal tubule all proteins were present in dots along the plasma membrane and most conspicuous below the brush border. However, clathrin and AP2 co-localized in vesicle subtypes distinct from those containing clathrin and AP1. In the distal tubule and in the cortical collecting duct all proteins were found in the apex of the cells; however, AP1 and Clint/EpsinR sh...Continue Reading

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Citations

Dec 7, 2006·Histochemistry and Cell Biology·Douglas J TaatjesJürgen Roth
Nov 1, 2007·Histochemistry and Cell Biology·Christian ZuberJürgen Roth
Jul 19, 2011·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Akiyoshi UezuHiroyuki Nakanishi

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