Clathrin-dependent entry of a gingipain adhesin peptide and Porphyromonas gingivalis into host cells.

Cellular Microbiology
Heike Boisvert, M J Duncan

Abstract

Porphyromonas gingivalis, a Gram-negative oral anaerobe, is associated with periodontitis, a disease that in some form affects up to 80% of the adult population in the USA. The organism interacts with gingival epithelium and surrounding tissue, and in this study we analysed interactions initiated by P. gingivalis and by a peptide derived from the adhesin domain of arg-gingipain A, a member of a family of surface cysteine proteinases. Recombinant peptide A44 blocked adherence of bacteria to host cell monolayers, and bound to components of the cell membrane fraction. In pull-down assays A44 associated with proteins involved in a clathrin-dependent endocytosis pathway. Inhibitor studies confirmed a role for clathrin, and confocal microscopy demonstrated that both A44-coated beads and intact bacteria colocalized with GFP-clathrin in host cells. Finally, we used siRNA to determine whether clathrin or caveolin-1 was involved in association of peptide and intact bacteria with host cells. Again, the results of these assays indicated that association of both A44 and P. gingivalis depended on the presence of clathrin, and support a working model in which A44 initiates a clathrin-dependent pathway that potentially leads to internalization...Continue Reading

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Citations

Apr 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Heike BoisvertMargaret J Duncan
Dec 19, 2009·Periodontology 2000·Atsuo AmanoKayoko Tsuda
Dec 19, 2009·Periodontology 2000·Gena D Tribble, Richard J Lamont
May 7, 2014·International Journal of Cancer. Journal International Du Cancer·Yang ChenYongzhang Luo
Oct 26, 2016·Cellular Microbiology·Eloise J O'Donoghue, Anne Marie Krachler
Jun 27, 2017·Frontiers in Microbiology·Arif Tasleem Jan
Jul 25, 2018·Journal of Alzheimer's Disease : JAD·Sebastian AguayoLuis Gerardo Aguayo
Jul 28, 2017·Journal of Oral Microbiology·Young-Jung JungBong-Kyu Choi

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