Clathrin exchange during clathrin-mediated endocytosis

The Journal of Cell Biology
X WuL E Greene

Abstract

During clathrin-mediated endocytosis, clathrin-coated pits invaginate to form clathrin-coated vesicles (CVs). Since clathrin-coated pits are planar structures, whereas CVs are spherical, there must be a structural rearrangement of clathrin as invagination occurs. This could occur through simple addition of clathrin triskelions to the edges of growing clathrin-coated pits with very little exchange occurring between clathrin in the pits and free clathrin in the cytosol, or it could occur through large scale exchange of free and bound clathrin. In the present study, we investigated this question by studying clathrin exchange both in vitro and in vivo. We found that in vitro clathrin in CVs and clathrin baskets do not exchange with free clathrin even in the presence of Hsc70 and ATP where partial uncoating occurs. However, surprisingly FRAP studies on clathrin-coated pits labeled with green fluorescent protein-clathrin light chains in HeLa cells show that even when endocytosis is blocked by expression of a dynamin mutant or depletion of cholesterol from the membrane, replacement of photobleached clathrin in coated pits on the membrane occurs at almost the same rate and magnitude as when endocytosis is occurring. Furthermore, very l...Continue Reading

References

Mar 1, 1992·Experimental Cell Research·S H HansenB van Deurs
Sep 1, 1991·The Journal of Cell Biology·S L Schmid, E Smythe
Jan 1, 1990·Annual Review of Cell Biology·B M Pearse, M S Robinson
Feb 1, 1989·The Journal of Cell Biology·J E Heuser, R G Anderson
Nov 1, 1994·The Journal of Cell Biology·H DamkeS L Schmid
Oct 1, 1993·Biophysical Journal·A J Jin, R Nossal
Dec 7, 1995·Nature·E UngewickellE Eisenberg
Jan 1, 1997·Annual Review of Biochemistry·S L Schmid
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·A SubtilT E McGraw
Jul 10, 1999·Science·M Marsh, H T McMahon
Nov 13, 1999·Nature Cell Biology·I GaidarovJ H Keen
Jan 8, 2000·The Journal of Biological Chemistry·T GreenerL E Greene
Mar 18, 2000·The Journal of Biological Chemistry·R JiangE Eisenberg
Jun 14, 2000·Current Opinion in Neurobiology·L BrodinO Shupliakov
Jul 11, 2000·European Journal of Cell Biology·A UmedaE Ungewickell
Aug 31, 2000·Annual Review of Biochemistry·T Kirchhausen
Nov 21, 2000·Current Biology : CB·W E GallT R Graham
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·T Kirchhausen

❮ Previous
Next ❯

Citations

May 16, 2006·Cellular and Molecular Life Sciences : CMLS·S J Royle
Sep 13, 2008·Histochemistry and Cell Biology·Daniel Wüstner, Nils J Faergeman
Feb 6, 2004·Journal of Molecular Biology·Corinne J SmithBarbara M F Pearse
Dec 4, 2003·Neurobiology of Aging·Thomas A BlanpiedMichael D Ehlers
May 10, 2002·Nature·John F PresleyJennifer Lippincott-Schwartz
Feb 8, 2011·Nature Cell Biology·Jeffrey B DoyonDavid G Drubin
Dec 21, 2005·Nature Reviews. Molecular Cell Biology·Michael G Roth
May 4, 2004·Nature Structural & Molecular Biology·Michael J RustXiaowei Zhuang
Jul 28, 2011·Integrative Biology : Quantitative Biosciences From Nano to Macro·Vyas RamananRavi Radhakrishnan
Jan 30, 2010·The Journal of Biological Chemistry·Victoria Prieto-EchagüeW Todd Miller
Sep 21, 2012·The Journal of Biological Chemistry·Lisa M BondJames R Sellers
Feb 18, 2003·Molecular Biology of the Cell·Xufeng WuLois E Greene
Oct 11, 2003·Molecular Biology of the Cell·Howard S MoskowitzTimothy A Ryan
Feb 4, 2005·Molecular Biology of the Cell·Howard S MoskowitzTimothy A Ryan
Aug 27, 2005·Molecular Biology of the Cell·Kanyan XiaoAndrew P Kowalczyk
Apr 25, 2008·Molecular Biology of the Cell·Dong-Won LeeLois E Greene
Apr 15, 2003·Journal of Virology·Luc SnyersDieter Blaas
Oct 7, 2003·Annual Review of Neuroscience·Venkatesh N Murthy, Pietro De Camilli
Oct 29, 2010·BMC Biochemistry·Victoria Prieto-EchagüeW Todd Miller
Aug 4, 2012·Journal of Nanobiotechnology·Ruthrotha B SelviTapas K Kundu
Apr 17, 2012·PLoS Biology·Marcus J TaylorChristien J Merrifield
Oct 5, 2013·The Journal of Chemical Physics·M Muthukumar, Ralph Nossal
Sep 5, 2013·Cellular and Molecular Life Sciences : CMLS·Fatima-Zahra Idrissi, María Isabel Geli
Feb 15, 2014·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Tobias TimmelSimone Spuler
Apr 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gilbert Di Paolo, Pietro De Camilli
Jun 1, 2006·Proceedings of the National Academy of Sciences of the United States of America·Lars HinrichsenErnst J Ungewickell
Apr 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ikuko FujiwaraJohn A Hammer
Aug 29, 2014·Molecular Biology of the Cell·Joe GroveMark Marsh
Apr 11, 2003·Molecular Biology of the Cell·Rosa PuertollanoJuan S Bonifacino
Mar 1, 2008·FEBS Letters·Martine Girard, Peter S McPherson
May 9, 2012·The Journal of Cell Biology·Andrew I ShevchukYuri E Korchev
Sep 25, 2012·Traffic·Yi LuoJames H Keen
Nov 6, 2015·Molecular Biology of the Cell·Jingzhen DingSusan Ferro-Novick
Apr 28, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Kenneth OfokansiConrad Coester
Feb 9, 2010·Journal of Controlled Release : Official Journal of the Controlled Release Society·Abdallah MakhlofHirofumi Takeuchi

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Methods Mentioned

BETA
confocal microscopy
fluorescence recovery
PCR

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