Clathrin-mediated endocytosis is required for ANE 30-100K-induced autophagy

Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology
Mei-Huei LinChing-Yu Yen

Abstract

We identified an autophagy-inducing areca nut (AN) ingredient (AIAI) in the 30-100 kDa fraction of AN extract (ANE 30-100K). This study was to analyze the role of endocytosis in ANE 30-100K-induced autophagy. We used benzyl alcohol, dynasore, and shRNA of clathrin and dynamin to assess whether ANE 30-100K-induced cytotoxicity and accumulation of microtubule-associated protein 1 light chain 3 (LC3)-II were affected in oral (OECM-1) and esophageal (CE81T/VGH) carcinoma cells. Both benzyl alcohol and dynasore effectively reduced ANE 30-100K-induced cytotoxicity and LC3-II accumulation in OECM-1 and CE81T/VGH cells. Downregulated protein expression of both clathrin and dynamin by their shRNA also significantly attenuated ANE 30-100K-induced elevation of LC3-II levels in CE81T/VGH cells. These results indicate that AIAI may be engulfed by cells through clathrin-mediated endocytosis, which promotes the execution of the following autophagy program.

References

Mar 22, 1995·Biochimica Et Biophysica Acta·M C GiocondiC Le Grimellec
Jun 3, 2006·Developmental Cell·Eric MaciaTomas Kirchhausen
Jul 6, 2007·Autophagy·Noboru Mizushima, Tamotsu Yoshimori
Apr 17, 2008·Methods in Enzymology·Tom KirchhausenHenry E Pelish
Sep 2, 2008·Autophagy·Mei-Huei LinYoung-Chau Liu
Nov 22, 2008·Biochimica Et Biophysica Acta·Virginia ToddeIda J van der Klei
Mar 26, 2009·Annual Review of Biochemistry·Gary J Doherty, Harvey T McMahon
Aug 6, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Chia-Chi WangTong-Rong Jan
Jan 24, 2012·Journal of Cell Science·Ghita GhislatErwin Knecht
Apr 27, 2012·Nature Reviews. Cancer·Eileen White
Jun 26, 2013·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Ching-Yu YenYoung-Chau Liu
Dec 25, 2013·Cell Research·Yuchen FengDaniel J Klionsky
May 3, 2014·Cold Spring Harbor Perspectives in Biology·Sharon A ToozeZvulun Elazar
May 27, 2014·BioMed Research International·Julia M I Barth, Katja Köhler
Aug 22, 2014·Journal of Extracellular Vesicles·Laura Ann MulcahyDavid Raul Francisco Carter
Apr 19, 2015·Cell Communication and Signaling : CCS·Giulio PretaI Martin Sheldon
May 29, 2015·PloS One·Ching-Yu YenYoung-Chau Liu
May 12, 2016·Experimental and Therapeutic Medicine·Jiang LiChang-Yun Fang
Jul 17, 2016·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Jay Gopal RayAmit Chattopadhyay
Nov 4, 2016·The EMBO Journal·Bruno AntonnySandra Schmid

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