Clathrin modulates vesicle scission, but not invagination shape, in yeast endocytosis

ELife
Wanda KukulskiMarko Kaksonen

Abstract

In a previous paper (Picco et al., 2015), the dynamic architecture of the protein machinery during clathrin-mediated endocytosis was visualized using a new live imaging and particle tracking method. Here, by combining this approach with correlative light and electron microscopy, we address the role of clathrin in this process. During endocytosis, clathrin forms a cage-like coat around the membrane and associated protein components. There is growing evidence that clathrin does not determine the membrane morphology of the invagination but rather modulates the progression of endocytosis. We investigate how the deletion of clathrin heavy chain impairs the dynamics and the morphology of the endocytic membrane in budding yeast. Our results show that clathrin is not required for elongating or shaping the endocytic membrane invagination. Instead, we find that clathrin contributes to the regularity of vesicle scission and thereby to controlling vesicle size.

References

Nov 29, 1985·Science·G S Payne, R Schekman
Jan 1, 1996·Journal of Structural Biology·J R KremerJ R McIntosh
Dec 20, 1996·The Journal of Biological Chemistry·D S ChuG S Payne
Aug 18, 1997·FEBS Letters·Harvey T McMahonC Smith
Nov 1, 2001·Annual Review of Cell and Developmental Biology·Frances M BrodskyD E Wakeham
Jul 27, 2005·Journal of Structural Biology·I F Sbalzarini, P Koumoutsakos
Sep 27, 2005·Journal of Structural Biology·David N Mastronarde
Jul 28, 2006·Molecular Biology of the Cell·Thomas M NewpherSandra K Lemmon
Mar 1, 2007·Methods in Cell Biology·Kent McDonald
Mar 19, 2008·The Journal of Cell Biology·Fatima-Zahra IdrissiMaría-Isabel Geli
Aug 12, 2008·Journal of Structural Biology·Sabine PruggnallerAchilleas S Frangakis
Jul 9, 2010·Molecular Biology of the Cell·Ji-Young YounBrenda J Andrews
Jan 5, 2011·The Journal of Cell Biology·Wanda KukulskiJohn A G Briggs
Oct 19, 2011·Proceedings of the National Academy of Sciences of the United States of America·Takuma KishimotoDavid G Drubin
Nov 16, 2011·Traffic·Iwona I Smaczynska-de RooijK R Ayscough
Apr 24, 2012·Nature Cell Biology·Philip N Dannhauser, Ernst J Ungewickell
Sep 6, 2012·Proceedings of the National Academy of Sciences of the United States of America·Fatima-Zahra IdrissiMaría Isabel Geli
Feb 13, 2015·ELife·Andrea PiccoMarko Kaksonen

Citations

Feb 8, 2018·Nature Reviews. Molecular Cell Biology·Marko Kaksonen, Aurélien Roux
Jun 1, 2018·Molecular Biology of the Cell·Andrea PiccoMarko Kaksonen
Jul 15, 2018·FEBS Letters·Michael M LacyJulien Berro
Oct 16, 2016·Molecular Microbiology·Z SchultzhausB D Shaw
Sep 5, 2018·Journal of Cell Science·Xingyun QiDaniël Van Damme
Apr 2, 2020·Current Opinion in Cell Biology·Kasey J Day, Jeanne C Stachowiak
Mar 9, 2021·Current Opinion in Cell Biology·Kem A Sochacki, Justin W Taraska

Methods Mentioned

BETA
electron microscopy
fluorescence
electron tomography
correlative microscopy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

Glut1 Deficiency

Glut1 deficiency, an autosomal dominant, genetic metabolic disorder associated with a deficiency of GLUT1, the protein that transports glucose across the blood brain barrier, is characterized by mental and motor developmental delays and infantile seizures. Follow the latest research on Glut1 deficiency with this feed.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Separation Anxiety

Separation anxiety is a type of anxiety disorder that involves excessive distress and anxiety with separation. This may include separation from places or people to which they have a strong emotional connection with. It often affects children more than adults. Here is the latest research on separation anxiety.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.