Claudin-1, -3, -4 and -7 gene expression analyses in canine prostate carcinoma and mammary tissue derived cell lines

Neoplasma
S C HammerHugo Murua Escobar

Abstract

Claudins (CLDNs) are transmembrane proteins localised in the cell membrane of epithelial cells composing a structural and functional component of the tight junction protein complexes. In canine tumors deregulations of the CLDN expression patterns were described immunohistochemically. Targeting of claudin proteins has further been evaluated to establish novel therapeutic approaches by directed claudin binding. Precondition for the development of claudin targeting approaches in canine cells is the possibility to characterise claudin expression specifically and the availability of claudin positive cell lines. Herein PCR/qPCR assays were established allowing a rapid qualitative and quantitative characterisation of CLDN-1, -3, -4 and -7 gene expression in canine cell lines and tissues. Further commercially available antibodies were used to verify CLDN gene expression on protein level by Western blots. The developed assays were used to analyse six canine cell lines derived from mammary and prostate tissue for their CLDN-1, -3, -4 and -7 expressions. The canine cell line DT08/40 (prostate transitional cell carcinoma) was used for the establishment of specific CLDNs -1, -3, -4 and -7PCR/qPCR. The designed assays were verified by amplic...Continue Reading

Citations

Oct 1, 2016·International Journal of Molecular Sciences·Susanne C HammerHugo Murua Escobar
Aug 25, 2018·Journal of the Science of Food and Agriculture·Diming WangJianxin Liu
Nov 11, 2019·International Journal of Molecular Sciences·Jan Torben SchilleHugo Murua Escobar
Oct 30, 2021·Frontiers in Veterinary Science·Chen MeiYanli Lyu

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