ClC-3 chloride channel prevents apoptosis induced by thapsigargin in PC12 cells

Apoptosis : an International Journal on Programmed Cell Death
H-N ZhangY-Y Guan

Abstract

Cell volume can be altered by two different ways, swelling and shrinkage. Cell swelling is regulated by volume-regulated Cl- channel (VRC). It is not well understood whether shrinkage is regulated by VRC. We previously found that antisense oligonucleotide specific to ClC-3 (ClC-3 antisense) prevented cell proliferation, which was related to cell swell volume regulation. In the present study, we further studied the role of ClC-3 Cl- channel in cell apoptosis which was related to cell shrinkage volume regulation by using antisense oligonucleotide specific to ClC-3 (ClC-3 antisense) and ClC-3 cDNA transfection techniques. We found that thapsigargin (TG), a specific inhibitor of the endoplasmic reticulum calcium ATPase, evoked apoptotic morphological changes (including cytoplasmic blebbing, condensation of nuclear chromatin, and the formation of apoptotic bodies), DNA laddering, and caspase-3 activation in PC12 cells (Pheochromocytoma-derived cell line). TG increased the cell apoptotic population with a decrease in cell viability. These effects were consistent with the decrease in endogenous ClC-3 protein expression, which was also induced by TG. Overexpression of ClC-3 significantly inhibited TG effect on PC12 cell apoptosis, wher...Continue Reading

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Citations

Mar 1, 2012·Histochemistry and Cell Biology·Jianwen MaoLixin Chen
Mar 3, 2007·Apoptosis : an International Journal on Programmed Cell Death·Song JinJia-Guo Zhou
Mar 5, 2011·Apoptosis : an International Journal on Programmed Cell Death·Yan QianYong-Yuan Guan
Jul 23, 2013·Apoptosis : an International Journal on Programmed Cell Death·Jing LiuYan-Hua Du
Mar 25, 2008·Biochemical Pharmacology·Jianwen MaoLiwei Wang
Oct 30, 2012·Acta Pharmacologica Sinica·Ya-ping ZhangDayue Darrel Duan
Feb 25, 2010·Clinical and Experimental Pharmacology & Physiology·Yong-Bo TangYong-Yuan Guan
Sep 2, 2009·Clinical and Experimental Pharmacology & Physiology·Xiao-Yan LiYong-Yuan Guan
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Dec 29, 2012·The International Journal of Biochemistry & Cell Biology·Haifeng ZhangLiwei Wang
Jan 18, 2011·The Journal of Pharmacy and Pharmacology·Ren-Hong DuYong-Yuan Guan
Apr 2, 2013·Biochimica Et Biophysica Acta·Nurit KeinanVarda Shoshan-Barmatz
Nov 26, 2014·Oncology Reports·Sen HongChunyan Zhao
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Jul 5, 2018·Cell Biology International·Lin-Yan HuangPing Ma
Mar 26, 2016·Cell Research·Kirby N Swatek, David Komander

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