PMID: 4290407Jun 1, 1966Paper

Clearing-factor lipase in adipose tissue. Factors influencing the increase in enzyme activity produced on incubation of tissue from starved rats in vitro

The Biochemical Journal
D R WingD S Robinson

Abstract

1. Evidence is presented that the increase in clearing-factor lipase activity that occurs when adipose tissue from starved rats is incubated in a defined medium in vitro is due to an increase in the total enzyme content of the system. It is shown that the clearing-factor lipase activity rises to reach a plateau level where, it is suggested, rates of enzyme synthesis and of enzyme destruction become balanced. 2. The presence of heparin in the incubation medium results in the extraction of part of the clearing-factor lipase originally present in the adipose tissue and this could provide the stimulus for the increase in total enzyme content. 3. Glucose is required in the incubation medium at a very low concentration. It can be replaced by fructose, but not by pyruvic acid, lactic acid, glyceric acid or dihydroxyacetone. 4. Adrenaline and corticotrophin inhibit the increase in enzyme activity when they are present in the incubation medium. 5. The high clearing-factor lipase activity associated with adipose tissue of fed rats is decreased by 50% within 3hr. of the injection of puromycin.

Citations

Nov 1, 1968·Journal of Atherosclerosis Research·J R Muir
Apr 12, 2014·Biochimica Et Biophysica Acta·Sander Kersten
Feb 1, 1971·The British Journal of Surgery·C M MorrisJ M Ham
Jul 1, 1969·The British Journal of Surgery·J M Ham, R W Furneaux
Mar 1, 1980·The British Journal of Nutrition·R Fears, E A Murrell
Oct 1, 1977·Scandinavian Journal of Clinical and Laboratory Investigation·H Lithell, J Boberg
May 1, 1977·Journal of Toxicology and Environmental Health·C R MackererM A Mehlman
Sep 4, 2020·Biological & Pharmaceutical Bulletin·Tomoyasu FujiiTetsuo Morita
Jan 1, 1974·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·G OehlerL Róka
Jan 24, 1975·Biochimica Et Biophysica Acta·D M Kornhauser, M Vaughan
Jul 18, 1986·Biochimica Et Biophysica Acta·K L BallD S Robinson
May 16, 1967·Biochimica Et Biophysica Acta·N PokrajacI L Chaikoff
Sep 28, 1978·Biochimica Et Biophysica Acta·I M SpencerD S Robinson
Sep 1, 1969·Clinica Chimica Acta; International Journal of Clinical Chemistry·J M Ham, W W Slack
Jul 16, 1982·Biochemical and Biophysical Research Communications·A M BourdeauxY Giudicelli
Jul 10, 1974·Biochemical and Biophysical Research Communications·C RaultG Sezille

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.