PMID: 11927677Apr 3, 2002Paper

Cleavage-arrested cell triplets from ascidian embryo differentiate into three cell types depending on cell combination and contact timing

The Journal of Physiology
Motoko Tanaka-Kunishima, Kunitaro Takahashi

Abstract

During early ascidian development, which is a prototype of early vertebrate development, anterior neuroectoderm cells (a4.2) from the eight-cell embryo are destined to become anterior neural structures including the brain vesicle, while presumptive notochordal neural cells (A4.1) become larval posterior neural structures including motoneurons. Whereas, an anterior quadrant cell (A3) of the four-cell embryo, from which both anterior neuroectoderm (a4.2) and notochordal neural cells (A4.1) are derived, has both fates. Cleavage-arrested cell triplets were prepared from the anterior quadrant cell and a pair of anterior neuroectoderm cells (A3-aa triplet) or a pair of presumptive notochordal neural cells (A3-AA triplet), and cultured in contact. Differentiation of cells in the triplet was determined electrophysiologically by observing cell type-specific currents. In the A3-aa triplet, when two neuroectoderm cells and an anterior quadrant cell were prepared from the same batch of embryos, all three cells in the triplet developed into neuronal cells in 60 % of cases, but in 40 % of cases all of them differentiated into epidermal cells. However, when the batch of embryos from which neuroectoderm cells were prepared was fertilized 3 h l...Continue Reading

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Citations

May 10, 2008·Neuroscience Letters·Biju ParekkadanMartin L Yarmush
Jul 27, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Motoko Tanaka-KunishimaFumiyuki Watanabe

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