PMID: 8600449Mar 1, 1996Paper

Cleavage by RNase P of gene N mRNA reduces bacteriophage lambda burst size

Nucleic Acids Research
Y Li, S Altman

Abstract

RNase P, an enzyme essential for tRNA biosynthesis, can be directed to cleave any RNA when the target RNA is in a complex with a short, complementary oligonucleotide called an external guide sequence (EGS). RNase P from Escherichia coli can cleave phage lambda N mRNA in vitro or in vivo when the mRNA is in a complex with an EGS. The EGS can either be separate from or covalently linked to M1 RNA, the catalytic RNA subunit of RNase P. The requirement for Mg2+ in the reaction in vitro is lower when the EGS is covalently linked to M1 RNA. Substrates made of DNA can also be cleaved by RNase P in vitro in complexes with RNA EGSs. When either kind of EGS construct is used in vivo, burst size of phage lambda is reduced by > or = 40%. Reduction in burst size depends on efficient expression of the EGS constructs. The product of phage lambda gene N appears to function in a stoichiometric fashion.

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Citations

Jul 15, 1996·Nucleic Acids Research·F Liu, S Altman
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·C Guerrier-TakadaS Altman
Jun 24, 1998·Proceedings of the National Academy of Sciences of the United States of America·D Plehn-Dujowich, S Altman
Nov 4, 2000·Expert Opinion on Investigational Drugs·H A James
Mar 10, 2010·New Biotechnology·Eirik Wasmuth Lundblad, Sidney Altman
Jan 27, 2010·Journal of Molecular Biology·William H McClainVenkat Gopalan
May 1, 1997·Human & Experimental Toxicology·G R SadaniG D Nadkarni
Jun 26, 2021·Frontiers in Bioengineering and Biotechnology·Victor Chinomso UjorThaddeus Chukwuemeka Ezeji

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