PMID: 9427530Jan 14, 1998Paper

Cleavage effect of oligoribonucleotides substituted at the cleavage sites with modified pyrimidine- and purine-nucleosides

Biochimica Et Biophysica Acta
K HosonoH Takaku

Abstract

The precursor of an RNA molecule from T4-infected E. coli cells (p2Spl RNA) has the capacity to cleave itself at specific positions [UpA (139-140) and CpA (170-171)], within a putative loop and stem structure. This sequence-specific cleavage requires at least a monovalent cation and non-ionic detergents. In order to determine the influence of the pyrimidine and purine bases on these sequence-specific cleavage reactions, we studied the cleavage reactions of hairpin loop RNAs substituted at the cleavage sites with modified pyrimidine- and purine-nucleosides. The cleavage was affected by the 2'-hydroxyl groups and the bases of the pyrimidines, and the 6-amino group of the purine.

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Citations

Jan 2, 2001·Journal of Inorganic Biochemistry·H HosakaH Takaku
Feb 15, 2000·Biochimica Et Biophysica Acta·K HosonoH Takaku
Sep 25, 2002·Biochemical and Biophysical Research Communications·Hidehiro TakahashiTakeshi Kurata

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