Cleavage-furrow formation without F-actin in Chlamydomonas.

Proceedings of the National Academy of Sciences of the United States of America
Masayuki OnishiJohn R Pringle

Abstract

It is widely believed that cleavage-furrow formation during cytokinesis is driven by the contraction of a ring containing F-actin and type-II myosin. However, even in cells that have such rings, they are not always essential for furrow formation. Moreover, many taxonomically diverse eukaryotic cells divide by furrowing but have no type-II myosin, making it unlikely that an actomyosin ring drives furrowing. To explore this issue further, we have used one such organism, the green alga Chlamydomonas reinhardtii We found that although F-actin is associated with the furrow region, none of the three myosins (of types VIII and XI) is localized there. Moreover, when F-actin was eliminated through a combination of a mutation and a drug, furrows still formed and the cells divided, although somewhat less efficiently than normal. Unexpectedly, division of the large Chlamydomonas chloroplast was delayed in the cells lacking F-actin; as this organelle lies directly in the path of the cleavage furrow, this delay may explain, at least in part, the delay in cytokinesis itself. Earlier studies had shown an association of microtubules with the cleavage furrow, and we used a fluorescently tagged EB1 protein to show that microtubules are still asso...Continue Reading

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Citations

Dec 1, 2020·Molecular Biology of the Cell·Prachee Avasthi
Jan 10, 2021·Biochimica Et Biophysica Acta. Molecular Cell Research·James BarrettLuke C M Mackinder
Apr 3, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Holly V GoodsonSusan H Brawley

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